{
  "abstract": "Background\n\nSuccinic acid (SA) biosynthesis presents a promising sustainable alternative to petrochemical routes, yet industrial potential is often hampered by inefficient CO₂ fixation and redox imbalances.Results\n\nTo systematically address these bottlenecks, a synergistic metabolic engineering strategy was developed in Actinobacillus succinogenes by co-expressing the endogenous gene of formate dehydrogenase (FdoG) and the heterologous gene of phosphoenolpyruvate carboxylase (PPC). This design established a self-reinforcing metabolic loop where FdoG-mediated formate oxidation generated in situ CO₂ and NADH, which were immediately channeled into the PPC-driven carboxylation pathway. Physiological characterizations, utilizing real-time off-gas analysis, demonstrated that this pathway effectively functions as an endogenous CO₂ generator, decoupling production from external mass transfer limitations. Furthermore, carbon flux distribution analysis confirmed the synergistic efficacy, revealing a 12.11% increase in succinate-directed flux and a significant reduction in byproduct accumulation compared to the wild type. To fully exploit this formate-utilization potential, an optimized fed-batch fermentation with pulsed formate feeding was implemented. Consequently, the engineered strain AS-PF achieved a remarkable succinate titer of 96.64 g/L, with a productivity of 1.38 g/ (L·h), corresponding to an overall product yield of 0.78 g/g based on the total consumed mixed carbon source (glucose and formate).Conclusion\n\nThis study establishes a sustainable and efficient platform for SA bioproduction by synergistically integrating formate metabolism-driven CO₂ recycling with enhanced biosynthetic pathways, thereby advancing the valorization of C1 compounds.",
  "abstract_shown": true,
  "affiliations": [
    "MOE Key Laboratoy of Bio-Intelligent Manufacturing, School of Bioengineering, Dalian University of Technology, No. 2 Linggong Road, Ganjingzi District, Dalian, 116024, Liaoning, People's Republic of China.",
    "MOE Key Laboratoy of Bio-Intelligent Manufacturing, School of Bioengineering, Dalian University of Technology, No. 2 Linggong Road, Ganjingzi District, Dalian, 116024, Liaoning, People's Republic of China. sunyaqin@dlut.edu.cn."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Mingyang Zhao"
    },
    {
      "affs": [
        0
      ],
      "name": "Li Wang"
    },
    {
      "affs": [
        0
      ],
      "name": "Yuanming Ye"
    },
    {
      "affs": [
        1
      ],
      "name": "Yaqin Sun"
    },
    {
      "affs": [
        0
      ],
      "name": "Zhilong Xiu"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/2a5p6bgznq319.bib",
  "cited_by": 0,
  "doi": "10.1186/s13068-026-02799-7",
  "fulltext_url": "https://europepmc.org/articles/PMC13685095",
  "id": "2a5p6bgznq319",
  "issns": [
    "2731-3654"
  ],
  "landing_url": "https://europepmc.org/article/MED/42902297",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "lcc",
      "TP248.13-248.65"
    ],
    [
      "lcc",
      "TP315-360"
    ],
    [
      "openalex",
      "1312|Microbial Metabolic Engineering and Bioproduction"
    ]
  ],
  "openalex": "W7171246400",
  "pdf_url": "https://europepmc.org/articles/PMC13685095?pdf=render",
  "pmcid": "PMC13685095",
  "pmid": "42902297",
  "published": "2026-07-01",
  "publisher": "BMC",
  "ris": "https://research.draconitic.com/paper/2a5p6bgznq319.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:41Z",
      "native_id": "MED:42902297",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42902297"
    },
    {
      "first_seen": "2026-10-30T13:45:45Z",
      "native_id": "6c213397cb6a4c45b039ad4259737e42",
      "source": "doaj",
      "url": "https://doi.org/10.1186/s13068-026-02799-7"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Microbial Metabolic Engineering and Bioproduction",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Formate-driven in situ CO₂ recycling for efficient succinic acid bioproduction in engineered Actinobacillus succinogenes.",
  "title_text": "Formate-driven in situ CO₂ recycling for efficient succinic acid bioproduction in engineered Actinobacillus succinogenes.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T13:45:45Z",
  "url": "https://research.draconitic.com/paper/2a5p6bgznq319",
  "venue": "Biotechnology for biofuels and bioproducts",
  "versions": []
}