{
  "abstract": "Patients with primary sclerosing cholangitis-associated ulcerative colitis (PSC-UC) have an elevated risk of colorectal cancer compared with ulcerative colitis (UC), but the molecular mechanisms linking chronic inflammation to epithelial cell fate remain unclear. The present study investigated disease- and region-specific molecular patterns involving miRNAs and associated PI3K/AKT, STAT3, and NF-κB signalling pathways. miR-21, miR-181a, miR-181b, and key pathway components were analysed in paired ascending and sigmoid colon biopsies from patients with PSC, PSC-UC, UC, and controls. Functional effects were examined in HT-29 and NCM460D cells, and circulating miRNAs, including miR-155, were assessed. UC was characterised by marked miR-181a upregulation and PI3K/AKT activation, whereas PSC-UC showed reduced PDCD4, divergent p65 regulation, and altered miR-181a/miR-181b coordination. In NCM460D cells, miR-181a and miR-181b reduced viability, promoted apoptosis, impaired cell-cycle progression, and decreased PDCD4, p-AKT, and p-STAT3; miR-181b additionally reduced CYLD. Serum miR-155 was elevated in PSC-UC compared with UC and showed the strongest individual discriminatory capacity. Random forest analysis and an exploratory multivariable serum miRNA profile showed discriminatory potential. These findings support disease- and region-specific molecular patterns involving miRNAs and associated signalling pathways linked to epithelial cell-fate responses in PSC-UC and highlight molecular differences between PSC-UC and conventional UC.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Functional Anatomy, Pomeranian Medical University in Szczecin, Powstańców Wielkopolskich 72, 70-111 Szczecin, Poland.",
    "Department of Medical Biology, Pomeranian Medical University in Szczecin, Powstańców Wielkopolskich 72, 70-111 Szczecin, Poland.",
    "Department of Gastroenterology, Pomeranian Medical University in Szczecin, Unii Lubelskiej 1, 71-252 Szczecin, Poland.",
    "Department of Clinical Anatomy, Pomeranian Medical University in Szczecin, Ku Słońcu 12, 71-073 Szczecin, Poland.",
    "Liver and Internal Medicine Unit, Department of General, Transplant and Liver Surgery, Medical University of Warsaw, 02-091 Warsaw, Poland.",
    "Translational Medicine Group, Pomeranian Medical University in Szczecin, 70-204 Szczecin, Poland."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Alicja Łaba",
      "orcid": "0009-0004-9544-4647"
    },
    {
      "affs": [
        1
      ],
      "name": "Joanna Abramczyk",
      "orcid": "0009-0005-4190-9901"
    },
    {
      "affs": [
        0
      ],
      "name": "Michał Lubkowski",
      "orcid": "0009-0007-4600-8920"
    },
    {
      "affs": [
        2
      ],
      "name": "Wiesława Rogoza-Mateja"
    },
    {
      "affs": [
        1
      ],
      "name": "Malgorzata Milkiewicz",
      "orcid": "0000-0002-2064-0545"
    },
    {
      "affs": [
        3
      ],
      "name": "Oktawian Knap"
    },
    {
      "affs": [
        4,
        5
      ],
      "name": "Piotr Milkiewicz"
    },
    {
      "affs": [
        0
      ],
      "name": "Agnieszka Kempinska-Podhorodecka",
      "orcid": "0000-0002-7513-8640"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/2c0f5hm03m3kb.bib",
  "cited_by": 0,
  "doi": "10.3390/cells15191821",
  "fulltext_url": "https://europepmc.org/articles/PMC13664130",
  "id": "2c0f5hm03m3kb",
  "issns": [
    "2073-4409"
  ],
  "landing_url": "https://europepmc.org/article/MED/42882519",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1311|Inflammatory Bowel Disease"
    ]
  ],
  "openalex": "W7220838972",
  "pdf_url": "https://europepmc.org/articles/PMC13664130?pdf=render",
  "pmcid": "PMC13664130",
  "pmid": "42882519",
  "published": "2026-10-07",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/2c0f5hm03m3kb.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:00Z",
      "native_id": "MED:42882519",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42882519"
    },
    {
      "first_seen": "2026-10-30T12:19:02Z",
      "native_id": "10.3390/cells15191821",
      "source": "crossref",
      "url": "https://www.mdpi.com/2073-4409/15/19/1821"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1311|Inflammatory Bowel Disease",
      "subfield": "genetics-genomics"
    }
  ],
  "title": "Disease- and Region-Specific Molecular Patterns Involving miRNAs and Associated Signalling Pathways and Epithelial Cell-Fate Responses in PSC-Associated Ulcerative Colitis.",
  "title_text": "Disease- and Region-Specific Molecular Patterns Involving miRNAs and Associated Signalling Pathways and Epithelial Cell-Fate Responses in PSC-Associated Ulcerative Colitis.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:02Z",
  "url": "https://research.draconitic.com/paper/2c0f5hm03m3kb",
  "venue": "Cells",
  "versions": []
}