{
  "abstract": "Red and near‐infrared emitting dyes are of high interest in fluorescence microscopy. Herein, we report two new red emissive dyes, namely RhodaMal 620 and RhodaMal 638 by incorporation of malononitrile, a highly electron‐withdrawing and π‐extending moiety into the traditional rhodol framework. The dyes have their absorption and emission maxima at 620/638 and 664/708 nm in aqueous solution. By increasing the viscosity of the medium to mimic the microenvironment of crowded and viscous subcellular compartments, we showed a drastic 20‐fold increase in the fluorescence signal. Conjugating the dyes to triphenylphosphonium cation (TPP+) enabled efficient mitochondrial targeting. RhodaMal 620 was further used in live cell STED microscopy with a 775 nm depletion laser, enabling super resolution with raw full‐width at half maximum values down to 77 nm.",
  "abstract_shown": true,
  "affiliations": [
    "Institute of Chemistry and Biochemistry Freie Universität Berlin Berlin Germany",
    "SupraFAB Freie Universität Berlin Berlin Germany",
    "Institute For Biology/Genetics Freie Universität Berlin Berlin Germany"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "A. Mavroskoufis"
    },
    {
      "affs": [
        0
      ],
      "name": "P. Aßmann"
    },
    {
      "affs": [
        0
      ],
      "name": "L. M. Filla"
    },
    {
      "affs": [
        0
      ],
      "name": "I. Bilici"
    },
    {
      "affs": [
        1
      ],
      "name": "E. Quaas"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "M. Maglione"
    },
    {
      "affs": [
        2
      ],
      "name": "S. Sigrist"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "R. Haag"
    },
    {
      "affs": [
        0
      ],
      "name": "K. Licha"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/2evq32763rbnk.bib",
  "cited_by": 0,
  "doi": "10.1002/chem.71410",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13715048/",
  "id": "2evq32763rbnk",
  "issns": [
    "0947-6539",
    "1521-3765"
  ],
  "landing_url": "https://doi.org/10.1002/chem.71410",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Research Article"
    ],
    [
      "openalex",
      "2505|Luminescence and Fluorescent Materials"
    ]
  ],
  "openalex": "W7169672470",
  "pdf_url": "https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/chem.71410",
  "pmcid": "PMC13715048",
  "pmid": "42470631",
  "published": "2026-07-18",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/2evq32763rbnk.ris",
  "sources": [
    {
      "first_seen": "2026-11-01T12:22:26Z",
      "native_id": "PMC13715048",
      "source": "pmc",
      "url": "https://doi.org/10.1002/chem.71410"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "openalex:2505|Luminescence and Fluorescent Materials",
      "subfield": "materials-polymers"
    }
  ],
  "title": "RhodaMal Dyes: Novel Red Emissive Fluorophores as Probes for High Contrast Live‐Cell Imaging",
  "title_text": "RhodaMal Dyes: Novel Red Emissive Fluorophores as Probes for High Contrast Live‐Cell Imaging",
  "type": "peer-reviewed",
  "updated_at": "2026-11-01T12:22:26Z",
  "url": "https://research.draconitic.com/paper/2evq32763rbnk",
  "venue": "Chemistry (Weinheim an Der Bergstrasse, Germany)",
  "versions": []
}