{
  "abstract": "Nipah virus (NiV) is a biosafety level 4 pathogen that has consistently posed a significant threat to public health. The two envelope proteins on the viral surface, the receptor-binding protein (RBP) and the fusion (F) protein, are the primary targets of neutralizing antibodies. However, the use of antibodies targeting a single viral antigen often leads to the emergence of resistant strains. Here, by panning phage display library, we obtained a C-type nanoantibody (C-Nab), G-3-1, targeting the head domain of RBP. The half-life-extended G-3-1-HLE effectively protected hamsters against authentic NiV challenge. Furthermore, we constructed a fusion protein, 2A1-3-8-(AP)12-G-3-1, by linking G-3-1 to the F-targeting nanobody 2A1-3-8 via a rigid (AP)12 linker. This fusion protein exhibited improved and balanced neutralizing activity. In addition, the in vivo prophylactic efficacy of 2A1-3-8-(AP)12-G-3-1 was validated in a mouse model of NiV pseudovirus infection. Collectively, this study on a dual-envelope-protein-targeting bispecific antibody not only provides a promising antiviral candidate, but also offers an important design strategy for next-generation antibody optimization.",
  "abstract_shown": true,
  "affiliations": [
    "State Key Laboratory of Virology and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China",
    "University of Chinese Academy of Sciences, Beijing 100049, China",
    "Department of Infectious Disease, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230027, China",
    "Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230027, China",
    "Key Laboratory of Anhui Province for Emerging and Reemerging Infectious Diseases, Hefei 230027, China"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 13,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Shengdong Wang"
    },
    {
      "affs": [
        0
      ],
      "name": "Xinghai Zhang"
    },
    {
      "affs": [
        0
      ],
      "name": "Haiwei Zhang"
    },
    {
      "affs": [
        0
      ],
      "name": "Guibo Rao"
    },
    {
      "affs": [
        0
      ],
      "name": "Yanfeng Yao"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Guihong Tian"
    },
    {
      "affs": [
        0
      ],
      "name": "Ge Gao"
    },
    {
      "affs": [
        0
      ],
      "name": "Yun Peng"
    },
    {
      "affs": [
        2,
        3,
        4
      ],
      "name": "Wujian Li"
    },
    {
      "affs": [
        0
      ],
      "name": "Sheng Cao"
    },
    {
      "affs": [
        2,
        3,
        4
      ],
      "name": "Sandra Chiu"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Rui Gong"
    },
    {
      "affs": [
        0
      ],
      "name": "Li Chen"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/2gsn6mw97xe3c.bib",
  "cited_by": 0,
  "doi": "10.1016/j.virs.2026.09.014",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13731425/",
  "id": "2gsn6mw97xe3c",
  "issns": [
    "1674-0769",
    "1995-820X"
  ],
  "landing_url": "https://doi.org/10.1016/j.virs.2026.09.014",
  "language": "en",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Research Article"
    ],
    [
      "openalex",
      "2713|Virology and Viral Diseases"
    ]
  ],
  "openalex": "W7221089103",
  "pdf_url": null,
  "pmcid": "PMC13731425",
  "pmid": "42849849",
  "published": "2026-10-09",
  "publisher": "Wuhan Institute of Virology, Chinese Academy of Sciences",
  "ris": "https://research.draconitic.com/paper/2gsn6mw97xe3c.ris",
  "sources": [
    {
      "first_seen": "2026-11-08T12:21:37Z",
      "native_id": "PMC13731425",
      "source": "pmc",
      "url": "https://doi.org/10.1016/j.virs.2026.09.014"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2713|Virology and Viral Diseases",
      "subfield": "epidemiology-public-health"
    }
  ],
  "title": "A dual-glycoprotein-targeting bispecific nanoantibody confers potent neutralization against Nipah virus",
  "title_text": "A dual-glycoprotein-targeting bispecific nanoantibody confers potent neutralization against Nipah virus",
  "type": "peer-reviewed",
  "updated_at": "2026-11-08T12:21:37Z",
  "url": "https://research.draconitic.com/paper/2gsn6mw97xe3c",
  "venue": "Virologica Sinica",
  "versions": []
}