{
  "abstract": "Pyrrolizidine alkaloids (PAs) are a chemically diverse group of plant-derived toxins whose quantification in botanical materials remains analytically challenging. This study integrates previously established nano zirconium silicate (NZS) enrichment with one-pot lithium aluminium hydride reduction/hydrolysis and LC–HRMS determination of the resulting necine-base response. The workflow targets retronecine- and heliotridine-type PAs and their N-oxides, with results expressed as retronecine equivalents. In Radix Primulae, three independent samples were spiked at each of 200, 250 and 300 µg/kg retronecine equivalents and processed through the complete analytical workflow. Mean recoveries were 83.0%, 94.2% and 92.5%, with between-preparation RSDs of 3.12%, 1.55% and 1.96%, respectively. Matrix-matched regression was evaluated using six calibration levels (10, 100, 200, 300, 400, 500; Day-1 R2 = 0.99936). Calculated Day-1 LOD and LOQ estimates were 14.94 and 45.27 µg/L. Remeasurement of the same extracts after 24 h, using a separate daily calibration, gave paired concentration changes of −0.67% to +1.23%. Application to a naturally contaminated comfrey sample demonstrated the workflow’s applicability beyond spiked samples, with agreement between the conversion-based result and the summed intact-PA reference approach.",
  "abstract_shown": true,
  "affiliations": [
    "ADSI—Austrian Drug Screening Institute GmbH, University of Innsbruck, Mitterweg 24, 6020 Innsbruck, Austria",
    "Institute of Analytical Chemistry and Radiochemistry, CCB—Center for Chemistry and Biomedicine, Leopold-Franzens University, Innrain 80/82, 6020 Innsbruck, Austria",
    "Bionorica SE, Kerschensteinerstraße 11–15, 92318 Neumarkt, Germany"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Benedikt Schwarz",
      "orcid": "0009-0009-2589-5828"
    },
    {
      "affs": [
        0
      ],
      "name": "Thomas Jakschitz",
      "orcid": "0000-0003-3699-3780"
    },
    {
      "affs": [
        1
      ],
      "name": "Christian W. Huck",
      "orcid": "0000-0002-6272-3242"
    },
    {
      "affs": [
        2
      ],
      "name": "Moritz Rubner"
    },
    {
      "affs": [
        2
      ],
      "name": "Michael Popp"
    },
    {
      "affs": [
        0
      ],
      "name": "Günther K. Bonn"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/2j507x5csm2ce.bib",
  "cited_by": 0,
  "doi": "10.3390/molecules31203590",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13707566/?tool=EBI",
  "id": "2j507x5csm2ce",
  "issns": [
    "1420-3049"
  ],
  "landing_url": "https://www.mdpi.com/1420-3049/31/20/3590",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Plant Toxicity and Pharmacological Properties"
    ]
  ],
  "openalex": "W7221158387",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13707566/pdf/?tool=EBI",
  "pmcid": "PMC13707566",
  "pmid": null,
  "published": "2026-10-01",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/2j507x5csm2ce.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:42Z",
      "native_id": "10.3390/molecules31203590",
      "source": "crossref",
      "url": "https://www.mdpi.com/1420-3049/31/20/3590"
    },
    {
      "first_seen": "2026-10-30T12:21:40Z",
      "native_id": "PMC:PMC13707566",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13707566"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Plant Toxicity and Pharmacological Properties",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Simplifying PA Quantification: A Unified Enrichment and Reduction Workflow for Retronecine-Based Quantification of Pyrrolizidine Alkaloids in Phyto Samples",
  "title_text": "Simplifying PA Quantification: A Unified Enrichment and Reduction Workflow for Retronecine-Based Quantification of Pyrrolizidine Alkaloids in Phyto Samples",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:21:40Z",
  "url": "https://research.draconitic.com/paper/2j507x5csm2ce",
  "venue": "Molecules",
  "versions": []
}