{
  "abstract": "Drug resistance and cancer stem cell maintenance drive poor outcomes in non-small cell lung cancer (NSCLC), yet the role of cancer-associated fibroblast (CAF)-derived exosomal circular RNAs remains insufficiently defined. This study investigated whether CAF-derived exosomal circTBPL1 regulates NSCLC stemness and cisplatin resistance through the miR-152-3p/Gli1 axis. Exosomal circRNAs from CAFs and normal fibroblasts were profiled by high-throughput sequencing, and circTBPL1 was validated using qRT-PCR, RNase R, actinomycin D, and fluorescence in situ hybridization. Functional assays, xenograft models, dual-luciferase reporter assays, RNA immunoprecipitation, RNA pull-down, rescue experiments, and TCGA analyses were performed. circTBPL1 was enriched in CAF-derived exosomes and displayed circular RNA stability and cytoplasmic localization. CAF-derived exosomal circTBPL1 promoted NSCLC cell proliferation, migration, invasion, tumor-sphere formation, stemness marker expression, and cisplatin resistance. circTBPL1 silencing in CAFs reduced cisplatin IC50 values, inhibited resistant-cell proliferation, and suppressed xenograft growth. Mechanistically, circTBPL1 acted as a competing endogenous RNA by binding miR-152-3p, relieving repression of Gli1 and activating Hedgehog-related resistance signaling. miR-152-3p inhibition or Gli1 overexpression partially reversed circTBPL1-knockdown effects. TCGA analysis linked high Gli1 expression with poorer survival in lung adenocarcinoma, supporting circTBPL1 as a potential biomarker candidate and therapeutic target requiring further clinical validation.",
  "abstract_shown": true,
  "affiliations": [
    "The Fourth Hospital of Hebei Medical University , , ,"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Yuan Mi"
    },
    {
      "affs": [
        0
      ],
      "name": "Ge Chen"
    },
    {
      "affs": [
        0
      ],
      "name": "Shifei Zhang"
    },
    {
      "affs": [
        0
      ],
      "name": "Yuxiang Liang"
    },
    {
      "affs": [
        0
      ],
      "name": "Fan Yu"
    },
    {
      "affs": [
        0
      ],
      "name": "Yaozhong Zhang",
      "orcid": "0000-0003-4986-4240"
    },
    {
      "affs": [
        0
      ],
      "name": "Kuankuan Li"
    },
    {
      "affs": [
        0
      ],
      "name": "Haidi Xu"
    },
    {
      "affs": [
        0
      ],
      "name": "Ziqiang Li"
    },
    {
      "affs": [
        0
      ],
      "name": "Lei Wang"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/2ket7ytv3qcpw.bib",
  "cited_by": 0,
  "doi": "10.1021/acsomega.6c07441",
  "fulltext_url": null,
  "id": "2ket7ytv3qcpw",
  "issns": [
    "2470-1343"
  ],
  "landing_url": "https://pubs.acs.org/acsodf/article/doi/10.1021/acsomega.6c07441/5465081/Cancer-Associated-Fibroblast-Derived-Exosomal",
  "language": null,
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [],
  "openalex": null,
  "pdf_url": "https://pubs.acs.org/doi/pdf/10.1021/acsomega.6c07441",
  "pmcid": null,
  "pmid": null,
  "published": "2026-11-05",
  "publisher": "American Chemical Society (ACS)",
  "ris": "https://research.draconitic.com/paper/2ket7ytv3qcpw.ris",
  "sources": [
    {
      "first_seen": "2026-11-06T00:21:08Z",
      "native_id": "10.1021/acsomega.6c07441",
      "source": "crossref",
      "url": "https://pubs.acs.org/acsodf/article/doi/10.1021/acsomega.6c07441/5465081/Cancer-Associated-Fibroblast-Derived-Exosomal"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "issn:2470-1343",
      "subfield": "general"
    }
  ],
  "title": "Cancer-Associated Fibroblast-Derived Exosomal circTBPL1 Regulates NSCLC Cell Stemness and Cisplatin Resistance via the miR-152-3p/Gli1 Axis",
  "title_text": "Cancer-Associated Fibroblast-Derived Exosomal circTBPL1 Regulates NSCLC Cell Stemness and Cisplatin Resistance via the miR-152-3p/Gli1 Axis",
  "type": "peer-reviewed",
  "updated_at": "2026-11-07T00:21:06Z",
  "url": "https://research.draconitic.com/paper/2ket7ytv3qcpw",
  "venue": "ACS Omega",
  "versions": []
}