{
  "abstract": "Skeletal muscle is essential for both movement and metabolic processes, characterized by a complex and ordered structure. Despite its importance, a detailed spatial map of gene expression within muscle tissue has been challenging to achieve due to the limitations of existing technologies, which struggle to provide high-resolution views. In this study, we leverage the Seq-Scope technique, an innovative method that allows for the observation of the entire transcriptome at an unprecedented submicron spatial resolution. By applying this technique to the mouse soleus muscle, we analyze and compare the gene expression profiles in both healthy conditions and following denervation, a process that mimics aspects of muscle aging. Our approach reveals detailed characteristics of muscle fibers, other cell types present within the muscle, and specific subcellular structures such as the postsynaptic nuclei at neuromuscular junctions, hybrid muscle fibers, and areas of localized expression of genes responsive to muscle injury, along with their histological context. The findings of this research significantly enhance our understanding of the diversity within the muscle cell transcriptome and its variation in response to denervation, a key factor in the decline of muscle function with age. This breakthrough in spatial transcriptomics not only deepens our knowledge of muscle biology but also sets the stage for the development of new therapeutic strategies aimed at mitigating the effects of aging on muscle health, thereby offering a more comprehensive insight into the mechanisms of muscle maintenance and degeneration in the context of aging and disease.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Molecular & Integrative Physiology, University of Michigan, Ann Arbor, MI, USA",
    "Space Planning and Analysis, University of Michigan, Ann Arbor, MI, USA",
    "Department of Biostatistics, University of Michigan, Ann Arbor, MI, USA",
    "Human Genetics Center, University of Texas Health Science Center at Houston, Houston, TX, USA"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 17,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Jer-En Hsu",
      "orcid": "0000-0002-2968-1853"
    },
    {
      "affs": [
        0
      ],
      "name": "Lloyd Ruiz"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Yongha Hwang",
      "orcid": "0000-0002-4345-581X"
    },
    {
      "affs": [
        0
      ],
      "name": "Steve D. Guzman",
      "orcid": "0000-0002-7934-7132"
    },
    {
      "affs": [
        0
      ],
      "name": "Chun‐Seok Cho",
      "orcid": "0000-0002-9589-5745"
    },
    {
      "affs": [
        2
      ],
      "name": "Weiqiu Cheng",
      "orcid": "0000-0003-4001-8662"
    },
    {
      "affs": [
        2
      ],
      "name": "Yichen Si",
      "orcid": "0000-0001-5576-3054"
    },
    {
      "affs": [
        0
      ],
      "name": "Peter C. D. Macpherson",
      "orcid": "0000-0002-2239-1204"
    },
    {
      "affs": [
        0
      ],
      "name": "Angelo Anacleto"
    },
    {
      "affs": [
        0,
        3
      ],
      "name": "Qingyang Zhao"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Xiaoya Zhao"
    },
    {
      "affs": [
        0
      ],
      "name": "Mitchell Schrank"
    },
    {
      "affs": [
        3,
        0
      ],
      "name": "Goo Jun",
      "orcid": "0000-0003-0891-0204"
    },
    {
      "affs": [
        2,
        0
      ],
      "name": "Hyun Min Kang",
      "orcid": "0000-0002-3631-3979"
    },
    {
      "affs": [
        0
      ],
      "name": "Myungjin Kim",
      "orcid": "0000-0002-3144-5235"
    },
    {
      "affs": [
        0
      ],
      "name": "Susan V. Brooks",
      "orcid": "0000-0003-1954-967X"
    },
    {
      "affs": [
        0
      ],
      "name": "Jun Hee Lee",
      "orcid": "0000-0002-2200-6011"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/2kkdg1b5r2wkp.bib",
  "cited_by": 12,
  "doi": "10.1111/febs.70726",
  "fulltext_url": null,
  "id": "2kkdg1b5r2wkp",
  "issns": [
    "1742-464X",
    "1742-4658"
  ],
  "landing_url": "https://doi.org/10.1111/febs.70726",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Single-cell and spatial transcriptomics"
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  "openalex": "W4392365712",
  "pdf_url": "https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/febs.70726",
  "pmcid": "PMC10925160",
  "pmid": "38464282",
  "published": "2026-09-21",
  "publisher": "Wiley",
  "ris": "https://research.draconitic.com/paper/2kkdg1b5r2wkp.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:26:46Z",
      "native_id": "W4392365712",
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      "url": "https://doi.org/10.1111/febs.70726"
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  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Single-cell and spatial transcriptomics",
      "subfield": "molecular-biology"
    }
  ],
  "title": "High‐resolution spatial transcriptomic atlas of mouse soleus muscle: Unveiling single cell and subcellular heterogeneity in health and denervation",
  "title_text": "High‐resolution spatial transcriptomic atlas of mouse soleus muscle: Unveiling single cell and subcellular heterogeneity in health and denervation",
  "type": "peer-reviewed",
  "updated_at": "2026-11-08T13:20:43Z",
  "url": "https://research.draconitic.com/paper/2kkdg1b5r2wkp",
  "venue": "FEBS Journal",
  "versions": []
}