{
  "abstract": "Amine-reactive Tandem Mass Tag 10plex (TMT10plex) labeling permits multiplexed protein identification and quantitative analysis by tandem mass spectrometry (MS/MS). We have used this technology to label 20 Saccharomyces cerevisiae samples collected in a time-resolved manner from a wild-type and phosphatase mutant background to characterize phosphoproteome dynamics. Here, we provide a detailed protocol for biological and mass spectrometry sample preparation and analysis. For complete details on the use and execution of this protocol, please refer to Touati et al. (2019).",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "name": "Andrew Jones"
    },
    {
      "name": "Helen Flynn"
    },
    {
      "name": "Frank Uhlmann"
    },
    {
      "name": "Ambrosius Snijders"
    },
    {
      "name": "Sandra Touati"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/2nh1agahsck76.bib",
  "cited_by": 17,
  "doi": "10.1016/j.xpro.2020.100022",
  "fulltext_url": null,
  "id": "2nh1agahsck76",
  "issns": [
    "2666-1667"
  ],
  "landing_url": "https://hal.science/hal-05750285v1",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "openalex",
      "1607|Advanced Proteomics Techniques and Applications"
    ]
  ],
  "openalex": "W3034140655",
  "pdf_url": "https://www.sciencedirect.com/science/article/pii/S2666166720300095/pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-15",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/2nh1agahsck76.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:34Z",
      "native_id": "hal-05750285",
      "source": "hal",
      "url": "https://hal.science/hal-05750285v1"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "openalex:1607|Advanced Proteomics Techniques and Applications",
      "subfield": "physical"
    }
  ],
  "title": "Assessing Budding Yeast Phosphoproteome Dynamics in a Time-Resolved Manner using TMT10plex Mass Tag Labeling",
  "title_text": "Assessing Budding Yeast Phosphoproteome Dynamics in a Time-Resolved Manner using TMT10plex Mass Tag Labeling",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:34Z",
  "url": "https://research.draconitic.com/paper/2nh1agahsck76",
  "venue": "STAR Protocols",
  "versions": []
}