{
  "abstract": "Fungal endophytes considered one of the major sources of promising bioactive compounds. This study aimed to isolate endophytic fungi from leaves of Reseda pruinosa for the first time, and to evaluate their biological activities. Results illustrated that, two fungal endophytes isolates were isolated, identified as Alternaria sp. and Alternaria alternata morphologically and genetically. Phytochemical analyses of these two fungal isolates metabolites showed significant amounts of phenolics, flavonoids, tannins, and alkaloids. GC-MS analyses of the crude extracts of these two fungal isolates showed that, major compounds in these extracts belong to the classes of fatty acid esters, fatty acids, and terpenoids. Results revealed that the MCF-7 cancer cells was susceptible to the anticancer effects of both fungal isolates crude extracts, with IC50 values of 96.3 and 117.4 µg/ml, respectively. Additionally, it showed promising antibacterial efficacy against pathogenic bacteria and fungi. Moreover, both crude extracts of fungal isolates exhibited antioxidant activity, with EC50 values of 234.8 µg/ml and 133.4 µg/ml respectively. Endophytic Alternaria species from leaves of R. pruinosa are a novel source for bioactive compounds that used in different medical fields.",
  "abstract_shown": true,
  "affiliations": [
    "Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt.",
    "Biology Department, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh, Saudi Arabia.",
    "Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia.",
    "Faculty of Science, Al-Azhar University, Cairo, Egypt."
  ],
  "article_type": "Research Article",
  "arxiv": null,
  "author_total": 15,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Amr H Hashem"
    },
    {
      "affs": [
        1
      ],
      "name": "Nosiba S Basher"
    },
    {
      "affs": [
        2
      ],
      "name": "Abeer S Aloufi"
    },
    {
      "affs": [
        0
      ],
      "name": "Salem S Salem",
      "orcid": "0000-0003-2898-6708"
    },
    {
      "affs": [
        3
      ],
      "name": "Mahmoud M Fawzi"
    },
    {
      "affs": [
        3
      ],
      "name": "Mohamed M Abdelaziz"
    },
    {
      "affs": [
        3
      ],
      "name": "Ahmed S Abdelrahman"
    },
    {
      "affs": [
        3
      ],
      "name": "Mohamed S Khader"
    },
    {
      "affs": [
        3
      ],
      "name": "Abdallah E Emam"
    },
    {
      "affs": [
        3
      ],
      "name": "Mohamed A Goma"
    },
    {
      "affs": [
        3
      ],
      "name": "Ahmed R Salama"
    },
    {
      "affs": [
        1
      ],
      "name": "Nasir A Ibrahim"
    },
    {
      "affs": [
        1
      ],
      "name": "Sulaiman A Alsalamah"
    },
    {
      "affs": [
        1
      ],
      "name": "Fahd A Nasr"
    },
    {
      "affs": [
        0
      ],
      "name": "Amer M Abdelaziz"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/2s5ha86m6rs5v.bib",
  "cited_by": 0,
  "doi": "10.1371/journal.pone.0357606",
  "fulltext_url": "https://europepmc.org/articles/PMC13718966",
  "id": "2s5ha86m6rs5v",
  "issns": [
    "1932-6203"
  ],
  "landing_url": "https://doi.org/10.1371/journal.pone.0357606",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Alternaria"
    ],
    [
      "heading",
      "Antibacterials"
    ],
    [
      "heading",
      "Antimicrobials"
    ],
    [
      "heading",
      "Antioxidants"
    ],
    [
      "heading",
      "Bacterial Pathogens"
    ],
    [
      "heading",
      "Biochemistry"
    ],
    [
      "heading",
      "Biology and Life Sciences"
    ],
    [
      "heading",
      "Chemical Compounds"
    ],
    [
      "heading",
      "Chemistry"
    ],
    [
      "heading",
      "Drugs"
    ],
    [
      "heading",
      "Eukaryota"
    ],
    [
      "heading",
      "Fungal Genetics"
    ],
    [
      "heading",
      "Fungal Pathogens"
    ],
    [
      "heading",
      "Fungi"
    ],
    [
      "heading",
      "Genetics"
    ],
    [
      "heading",
      "Medical Microbiology"
    ],
    [
      "heading",
      "Medicine and Health Sciences"
    ],
    [
      "heading",
      "Microbial Control"
    ],
    [
      "heading",
      "Microbial Pathogens"
    ],
    [
      "heading",
      "Microbiology"
    ],
    [
      "heading",
      "Mycology"
    ],
    [
      "heading",
      "Organisms"
    ],
    [
      "heading",
      "Pathogens"
    ],
    [
      "heading",
      "Pathology and Laboratory Medicine"
    ],
    [
      "heading",
      "Pharmacology"
    ],
    [
      "heading",
      "Phenols"
    ],
    [
      "heading",
      "Physical Sciences"
    ],
    [
      "heading",
      "Research Article"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Antioxidants"
    ],
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      "plos",
      "/Biology and life sciences/Genetics/Fungal genetics"
    ],
    [
      "plos",
      "/Biology and life sciences/Microbiology/Medical microbiology/Microbial pathogens/Bacterial pathogens"
    ],
    [
      "plos",
      "/Biology and life sciences/Microbiology/Medical microbiology/Microbial pathogens/Fungal pathogens"
    ],
    [
      "plos",
      "/Biology and life sciences/Microbiology/Medical microbiology/Microbial pathogens/Fungal pathogens/Alternaria"
    ],
    [
      "plos",
      "/Biology and life sciences/Microbiology/Microbial control/Antimicrobials/Antibacterials"
    ],
    [
      "plos",
      "/Biology and life sciences/Mycology/Fungal genetics"
    ],
    [
      "plos",
      "/Biology and life sciences/Mycology/Fungal pathogens"
    ],
    [
      "plos",
      "/Biology and life sciences/Mycology/Fungal pathogens/Alternaria"
    ],
    [
      "plos",
      "/Biology and life sciences/Organisms/Eukaryota/Fungi"
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      "plos",
      "/Medicine and health sciences/Pathology and laboratory medicine/Pathogens/Microbial pathogens/Bacterial pathogens"
    ],
    [
      "plos",
      "/Medicine and health sciences/Pathology and laboratory medicine/Pathogens/Microbial pathogens/Fungal pathogens"
    ],
    [
      "plos",
      "/Medicine and health sciences/Pathology and laboratory medicine/Pathogens/Microbial pathogens/Fungal pathogens/Alternaria"
    ],
    [
      "plos",
      "/Medicine and health sciences/Pharmacology/Drugs/Antimicrobials/Antibacterials"
    ],
    [
      "plos",
      "/Physical sciences/Chemistry/Chemical compounds/Phenols"
    ]
  ],
  "openalex": null,
  "pdf_url": "https://europepmc.org/articles/PMC13718966?pdf=render",
  "pmcid": "PMC13718966",
  "pmid": "42955937",
  "published": "2026-10-29",
  "publisher": "Public Library of Science",
  "ris": "https://research.draconitic.com/paper/2s5ha86m6rs5v.ris",
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  "subjects": [
    {
      "field": "biochemistry",
      "origin": "heading:Biochemistry",
      "subfield": "general"
    },
    {
      "field": "biology",
      "origin": "heading:Genetics",
      "subfield": "genetics-genomics"
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    {
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      "origin": "heading:Pharmacology",
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  ],
  "title": "Phytochemical analyses, antimicrobial, antioxidant and anticancer activities of endophytic fungi from leaves of Reseda pruinosa",
  "title_text": "Phytochemical analyses, antimicrobial, antioxidant and anticancer activities of endophytic fungi from leaves of Reseda pruinosa",
  "type": "peer-reviewed",
  "updated_at": "2026-11-02T12:22:53Z",
  "url": "https://research.draconitic.com/paper/2s5ha86m6rs5v",
  "venue": "PLOS One",
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}