{
  "abstract": "Failures in germline development drive male infertility, but the lack of model systems that recapitulate human spermatogenesis hampers therapeutic development. Here, we develop a system to differentiate human-induced pluripotent stem cells (iPSCs) into primordial germ cell-like cells that self-organize with mouse fetal testicular cells into seminiferous tubule-like structures within a xenogeneic reconstituted testis (xrTestis). Subsequent transplantation of xrTestes into immunodeficient mice results in efficient generation of male germ cells up to meiotic onset, including spermatogonia with evidence of prepachytene PIWI-interacting RNA (piRNA) biogenesis, as well as differentiating spermatogonia and rare preleptotene spermatocytes exhibiting transcriptomic and phenotypic similarities to their in vivo counterparts. As future clinical applications will require testing in non-human primates, we apply a similar strategy to differentiate rhesus iPSCs through fetal germ cell stages into spermatogonia and differentiating spermatogonia. Together, these platforms provide a foundation for studying primate germ cell lineages and represent a step toward in vitro gametogenesis.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Biomedical Sciences, School of Veterinary Medicine, University of Pennsylvania, 3800 Spruce Street, Philadelphia, PA, USA. Electronic address: ewhelan@upenn.edu",
    "Department of Biomedical Sciences, School of Veterinary Medicine, University of Pennsylvania, 3800 Spruce Street, Philadelphia, PA, USA",
    "Department of Biomedical Sciences, School of Veterinary Medicine, University of Pennsylvania, 3800 Spruce Street, Philadelphia, PA, USA; Department of Obstetrics and Gynecology, Yamaguchi University Graduate School of Medicine, Ube, Japan",
    "Regensburg Center for Biochemistry (RCB), Laboratory for RNA Biology, University of Regensburg, Regensburg, Germany; National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA",
    "National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Eoin Christopher Whelan",
      "orcid": "0000-0003-0842-7722"
    },
    {
      "affs": [
        1
      ],
      "name": "Mingyue Guo",
      "orcid": "0000-0002-3582-446X"
    },
    {
      "affs": [
        1
      ],
      "name": "Ryo Yokomizo",
      "orcid": "0000-0001-8161-8053"
    },
    {
      "affs": [
        1
      ],
      "name": "Yasunari Seita",
      "orcid": "0000-0002-5020-1594"
    },
    {
      "affs": [
        1
      ],
      "name": "Young Sun Hwang",
      "orcid": "0000-0002-6443-9579"
    },
    {
      "affs": [
        2
      ],
      "name": "Yuichiro Shirafuta",
      "orcid": "0000-0001-9150-0577"
    },
    {
      "affs": [
        1
      ],
      "name": "Nicolae Adrian Leu"
    },
    {
      "affs": [
        1
      ],
      "name": "Keren Cheng",
      "orcid": "0000-0002-9617-3584"
    },
    {
      "affs": [
        3
      ],
      "name": "Franziska Ahrend",
      "orcid": "0009-0004-7464-3444"
    },
    {
      "affs": [
        4
      ],
      "name": "Astrid D. Haase",
      "orcid": "0000-0003-1120-9013"
    },
    {
      "name": "Kotaro Sasaki",
      "orcid": "0000-0002-5604-2651"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/2venjw7ehym0d.bib",
  "cited_by": 9,
  "doi": "10.1016/j.stem.2026.06.001",
  "fulltext_url": null,
  "id": "2venjw7ehym0d",
  "issns": [
    "1875-9777",
    "1934-5909"
  ],
  "landing_url": "https://doi.org/10.1016/j.stem.2026.06.001",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Pluripotent Stem Cells Research"
    ]
  ],
  "openalex": "W7167921968",
  "pdf_url": null,
  "pmcid": null,
  "pmid": "42431197",
  "published": "2026-07-10",
  "publisher": "Elsevier BV",
  "ris": "https://research.draconitic.com/paper/2venjw7ehym0d.ris",
  "sources": [
    {
      "first_seen": "2026-11-04T13:21:07Z",
      "native_id": "W7167921968",
      "source": "openalex",
      "url": "https://doi.org/10.1016/j.stem.2026.06.001"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Pluripotent Stem Cells Research",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Generation of spermatogonia from human and non-human primate pluripotent stem cells",
  "title_text": "Generation of spermatogonia from human and non-human primate pluripotent stem cells",
  "type": "peer-reviewed",
  "updated_at": "2026-11-06T13:20:30Z",
  "url": "https://research.draconitic.com/paper/2venjw7ehym0d",
  "venue": "Cell stem cell",
  "versions": []
}