{
  "abstract": "Background/Objectives: Adenosine 5'-O-(β-thio)-diphosphate (ADPβS) is a stable analogue of adenosine diphosphate (ADP). In pithed rats, ADPβS produces (i) inhibition of both the cardioaccelerator sympathetic tone (via prejunctional P2Y₁₂/P2Y₁₃ receptors) and the vasodepressor sensory CGRPergic tone (via prejunctional P2Y₁ receptors) and (ii) vasodepressor/vasodilator responses via endothelial P2Y₁ receptors, and vasopressor/vasoconstrictor responses via P2Y₁/P2Y₁₂/P2Y₁₃ receptors. This study pharmacologically investigates whether P2Y₁, P2Y₁₂, and/or P2Y₁₃ receptors modulate the vasopressor sympathetic tone using ADPβS. Methods: A total of 126 male Wistar rats were pithed and classified into two groups to produce vasopressor responses by (i) spinal (T₇-T₉) electrical stimulation of the sympathetic vasopressor tone (0.03-3 Hz; n = 102) and (ii) intravenous (i.v.) boluses of noradrenaline (0.03-3 μg/kg; n = 24). Results: Continuous i.v. infusions of ADPβS (5.6 and 10 μg/kg·min) significantly inhibited the vasopressor responses to sympathetic electrical stimulation, without affecting those induced by exogenous noradrenaline. This sympathoinhibition by ADPβS (5.6 μg/kg·min) remained unchanged after vehicle (1 mL/kg, i.v.) or the antagonists PSB 0739 (P2Y12; 300 μg/kg, i.v.) and MRS 2211 (P2Y13; 1000 and 3000 μg/kg, i.v.). Conversely, it was abolished after MRS 2500 (P2Y1; 300 μg/kg, i.v.) or glibenclamide (KATP channel blocker; 20 mg/kg, i.v.). Conclusions: ADPβS inhibits the vasopressor sympathetic tone via prejunctional P2Y₁ receptors through KATP channel-dependent mechanisms.",
  "abstract_shown": true,
  "affiliations": [
    "Departamento de Farmacobiología, Cinvestav-Coapa, Czda. de los Tenorios 235, Col. Granjas Coapa, Deleg. Tlalpan, Ciudad de Mexico C.P. 14330, Mexico.",
    "Translational Research Centre, Copenhagen University Hospital-Rigshospitalet, Nordstjernevej 42, 2600 Glostrup, Denmark.",
    "Department of Health Technology, Technical University of Denmark, 2800 Kongens Lyngby, Denmark.",
    "Danish Headache Center, Department of Neurology, Copenhagen University Hospital-Rigshospitalet, 2600 Glostrup, Denmark.",
    "Division of Vascular Medicine and Pharmacology, Erasmus MC University Medical Center Rotterdam, P.O. Box 2040, 3000 CA Rotterdam, The Netherlands.",
    "Laboratorio de Farmacología, Departamento de Fisiología y Farmacología, Facultad de Farmacia, Universidad de Salamanca, Campus Miguel de Unamuno, C. Lic. Méndez Nieto, s/n, 37007 Salamanca, Spain."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Carlos M Villalón",
      "orcid": "0000-0003-4117-1343"
    },
    {
      "affs": [
        0
      ],
      "name": "Juan Linares-Bedolla",
      "orcid": "0000-0002-8968-8088"
    },
    {
      "affs": [
        0
      ],
      "name": "Belinda Villanueva-Castillo"
    },
    {
      "affs": [
        0
      ],
      "name": "Alejandro D Miguel-Martínez",
      "orcid": "0000-0001-5176-5466"
    },
    {
      "affs": [
        1,
        2,
        3
      ],
      "name": "Kristian A Haanes",
      "orcid": "0000-0001-5182-8957"
    },
    {
      "affs": [
        4
      ],
      "name": "Antoinette MaassenVanDenBrink",
      "orcid": "0000-0001-7176-126X"
    },
    {
      "affs": [
        5
      ],
      "name": "Juan F Fernández-González",
      "orcid": "0000-0002-2344-4523"
    },
    {
      "affs": [
        4
      ],
      "name": "Eduardo Rivera-Mancilla",
      "orcid": "0000-0003-4559-4028"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/2wkjqz9vje0h1.bib",
  "cited_by": 0,
  "doi": "10.3390/ph19101594",
  "fulltext_url": "https://europepmc.org/articles/PMC13708566",
  "id": "2wkjqz9vje0h1",
  "issns": [
    "1424-8247"
  ],
  "landing_url": "https://europepmc.org/article/MED/42943291",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1314|Adenosine and Purinergic Signaling"
    ]
  ],
  "openalex": "W7220910616",
  "pdf_url": "https://europepmc.org/articles/PMC13708566?pdf=render",
  "pmcid": "PMC13708566",
  "pmid": "42943291",
  "published": "2026-10-08",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/2wkjqz9vje0h1.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:22:24Z",
      "native_id": "MED:42943291",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42943291"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:1314|Adenosine and Purinergic Signaling",
      "subfield": "physiology"
    }
  ],
  "title": "Pharmacological Analysis of P2Y Purinergic Receptors That Modulate the Rat Vasopressor Sympathetic Tone.",
  "title_text": "Pharmacological Analysis of P2Y Purinergic Receptors That Modulate the Rat Vasopressor Sympathetic Tone.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:22:24Z",
  "url": "https://research.draconitic.com/paper/2wkjqz9vje0h1",
  "venue": "Pharmaceuticals (Basel, Switzerland)",
  "versions": []
}