{
  "abstract": "Nontuberculous mycobacterial pulmonary disease (NTM-PD) is rising globally, driven predominantly by Mycobacterium avium complex (MAC), yet treatment is highly species-dependent. We developed a Multiplex assay targeting four clinically important non-tuberculous mycobacteria (NTM) species– M. avium , M. intracellulare , M. abscessus , and M. fortuitum –and compared it with metagenomic next-generation sequencing (mNGS), using colony-based multigene Sanger sequencing as the reference method. Among 101 specimens, 63 (62.4%) yielded polymicrobial results, of which mixed-species detection of M. avium – M. intracellulare accounted for 46 (73.0%). The Multiplex assay and mNGS showed no statistically significant difference in overall accuracy in this cohort (82.2% vs. 81.2%; raw agreement 83.2%, P = 1.000). The Multiplex assay achieved 100.0% sensitivity for all four species, with specificity of 87.8% and 87.5% for M. avium and M. intracellulare , respectively, and >98% for M. abscessus and M. fortuitum ; mNGS showed the converse pattern, with lower MAC sensitivity (88.5%, 92.2%) but higher MAC specificity (93.9%, 95.8%). Requiring only a single PCR reaction without sequencing or bioinformatic analysis, the Multiplex assay showed no statistically significant difference in diagnostic accuracy from mNGS in this culture-positive cohort, without requiring library preparation or sequencing, representing a promising candidate for further evaluation as a routine NTM species-identification tool.",
  "abstract_shown": true,
  "affiliations": [
    "Shengli Clinical Medical College of Fujian Medical University, Fujian Medical University",
    "Tuberculosis Laboratory, International Medical Examination Center, Fuzhou University Affiliated Provincial Hospital",
    "Department of Clinical Laboratory, Fuzhou University Affiliated Provincial Hospital",
    "Department of Health Management, Fuzhou University Affiliated Provincial Hospital"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Zhen Li"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Yaocheng Wang"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Li Lai"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Yi Huang"
    },
    {
      "affs": [
        0,
        1,
        3
      ],
      "name": "Li Li"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/2x3zcqazhsn4z.bib",
  "cited_by": 0,
  "doi": "10.3389/fmicb.2026.1966137",
  "fulltext_url": "https://europepmc.org/articles/PMC13687058",
  "id": "2x3zcqazhsn4z",
  "issns": [
    "1664-302X"
  ],
  "landing_url": "https://www.frontiersin.org/articles/10.3389/fmicb.2026.1966137/full",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2713|Mycobacterium research and diagnosis"
    ]
  ],
  "openalex": "W7220986924",
  "pdf_url": "https://europepmc.org/articles/PMC13687058?pdf=render",
  "pmcid": "PMC13687058",
  "pmid": "42923191",
  "published": "2026-10-08",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/2x3zcqazhsn4z.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:12Z",
      "native_id": "10.3389/fmicb.2026.1966137",
      "source": "crossref",
      "url": "https://www.frontiersin.org/articles/10.3389/fmicb.2026.1966137/full"
    },
    {
      "first_seen": "2026-10-30T12:20:36Z",
      "native_id": "MED:42923191",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42923191"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2713|Mycobacterium research and diagnosis",
      "subfield": "epidemiology-public-health"
    }
  ],
  "title": "A novel multiplex assay for species-level identification of non-tuberculous mycobacteria in culture-positive respiratory specimens based on capillary electrophoresis: development and clinical validation",
  "title_text": "A novel multiplex assay for species-level identification of non-tuberculous mycobacteria in culture-positive respiratory specimens based on capillary electrophoresis: development and clinical validation",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:20:36Z",
  "url": "https://research.draconitic.com/paper/2x3zcqazhsn4z",
  "venue": "Frontiers in Microbiology",
  "versions": []
}