{
  "abstract": "Background: Primary chemotherapy refractoriness remains a clinical challenge in high-grade serous ovarian carcinoma (HGSC) and may reflect pre-existing tumor-cell states and microenvironmental heterogeneity. We examined public single-cell and bulk transcriptomic resources, a barcode-matched MRI case, and an in vitro cisplatin-perturbation RT-qPCR experiment.\n\nMethods: We reanalyzed GSE300897, comprising 19,156 annotated cells from nine treatment-naïve HGSC biopsy libraries (four chemotherapy-refractory and five chemotherapy-sensitive), with response summaries retained at the library/patient level. The Cancer Genome Atlas Ovarian Cancer (TCGA-OV) provided 421 unique primary tumors for cohort-scale transcriptomic and overall survival context. One TCGA-OV case (TCGA-24-1423) linked a multiparametric MRI examination to a tumor RNA-seq barcode. OVCAR-3 and Caov-3 cells were exposed to cisplatin (5 μM, 24 h), and IFIT1, MX1, SNAI1, and HIF1A expression was measured by RT-qPCR.\n\nResults: Across the nine GSE300897 libraries, no cancer-cell program passed Benjamini–Hochberg (BH) q < 0.05. Refractory libraries showed a lower type-I interferon (IFN-I) score (−0.15 log1p(CP10k)) and epithelial-to-mesenchymal transition (EMT) balance (−0.16), with a small increase in hypoxia (+0.05). In TCGA-OV, none of the six displayed program scores reached BH q < 0.05 in age- and stage-adjusted Cox models; IFN-I tertiles separated nominally by Kaplan–Meier analysis (log-rank p = 0.029), whereas continuous IFN-I was not significant after adjustment (HR = 0.96 per SD, BH q = 0.927). In TCGA-24-1423, the paired transcriptome showed an IFN-I score at the 86.8th TCGA-OV percentile, with IFIT1 and MX1 at the 87.5th and 93.7th percentiles. RT-qPCR showed lower IFIT1/MX1 and higher SNAI1/HIF1A after acute cisplatin exposure in both cell lines (all Holm-adjusted p < 0.05).\n\nConclusion: These findings support a conservative, hypothesis-generating interpretation of chemotherapy response-associated transcriptional heterogeneity in HGSC. Reduced IFN-I and altered cell-state programs were directional features of the small refractory single-cell cohort, whereas TCGA-OV provided context rather than independent response validation. The MRI-linked case demonstrates imaging–transcriptome pairing but does not establish an imaging biomarker. The RT-qPCR experiment demonstrates cisplatin-responsive changes in selected genes but models acute drug perturbation rather than the pretreatment clinical refractory state.",
  "abstract_shown": true,
  "affiliations": [
    "Radiology Imaging Center, Nanbai Xiang Hospital Complex, The First Affiliated Hospital of Wenzhou Medical University , , ,",
    "Gynecology Department, The First Affiliated Hospital of Wenzhou Medical University , , ,"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Litian Chen"
    },
    {
      "affs": [
        0
      ],
      "name": "Pingping Fu"
    },
    {
      "affs": [
        0
      ],
      "name": "Sunjie Wu"
    },
    {
      "affs": [
        0
      ],
      "name": "Ben Chen"
    },
    {
      "affs": [
        1
      ],
      "name": "Xin Wen"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/2x8j4eq363cgy.bib",
  "cited_by": 0,
  "doi": "10.3389/fgene.2026.1943981",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13724571/?tool=EBI",
  "id": "2x8j4eq363cgy",
  "issns": [
    "1664-8021"
  ],
  "landing_url": "https://europepmc.org/article/PMC/PMC13724571",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Original Research"
    ],
    [
      "openalex",
      "2743|Ovarian cancer diagnosis and treatment"
    ]
  ],
  "openalex": "W7222964886",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13724571/pdf/?tool=EBI",
  "pmcid": "PMC13724571",
  "pmid": "42973797",
  "published": "2026-10-19",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/2x8j4eq363cgy.ris",
  "sources": [
    {
      "first_seen": "2026-11-03T15:20:51Z",
      "native_id": "PMC:PMC13724571",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13724571"
    },
    {
      "first_seen": "2026-11-04T12:23:07Z",
      "native_id": "PMC13724571",
      "source": "pmc",
      "url": "https://doi.org/10.3389/fgene.2026.1943981"
    },
    {
      "first_seen": "2026-11-04T15:20:48Z",
      "native_id": "MED:42973797",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42973797"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2743|Ovarian cancer diagnosis and treatment",
      "subfield": "reproductive-child"
    }
  ],
  "title": "Chemotherapy-refractory high-grade serous ovarian carcinoma: single-cell findings, TCGA-OV transcriptomic context, and a single-case MRI–transcriptome illustration",
  "title_text": "Chemotherapy-refractory high-grade serous ovarian carcinoma: single-cell findings, TCGA-OV transcriptomic context, and a single-case MRI–transcriptome illustration",
  "type": "peer-reviewed",
  "updated_at": "2026-11-06T12:21:01Z",
  "url": "https://research.draconitic.com/paper/2x8j4eq363cgy",
  "venue": "Frontiers in genetics",
  "versions": []
}