{
  "abstract": "Extracellular vesicles (EVs) have the potential to be used as a liquid biopsy for cancer detection and treatment response assessment. Although the potential of EVs as disease-specific biomarkers has been promising, the rapid and specific enrichment of EVs from body fluids in a clinical setting remains challenging. To address this limitation, we developed a microfluidic electrophoresis (MEP) device for the label-free, charge-based enrichment of EVs from the serum of patients with pancreatic cancer (PaCa). We first validated the principle of charge-based cancer EV enrichment using cell-line-derived EVs, demonstrating that increased signaling through mutant Kirsten rat sarcoma viral oncogene homologue (KRAS), particularly KrasG12D, was associated with enhanced EV secretion and a shift toward more negative ζ-potential values. We then evaluated EVs derived from the serum of patients with PaCa to assess the clinical relevance and translational potential of the MEP platform. Further analyses identified phosphatidylserine and extraluminal DNA as part of the molecular determinants of the enhanced anionic nature of the PaCa-derived EVs. Overall, this proof-of-concept study introduces a promising microfluidic platform for enriching circulating cancer EVs with potential applications in the rapid detection of PaCa.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Cancer Biology, The University of Texas MD Anderson Cancer Center, Houston, Texas77030, United States.",
    "Department of Bioengineering, Rice University, Houston, Texas77005, United States.",
    "Department of Electrical and Computer Engineering, Rice University, Houston, Texas77005, United States.",
    "Department of Systems, Synthetic, and Physical Biology, Rice University, Houston, Texas77005, United States.",
    "Applied Physics Program, Rice University, Houston, Texas77005, United States.",
    "Department of Chemical and Biomolecular Engineering, Rice University, Houston, Texas77005, United States.",
    "Department of Surgery II, University of Witten/Herdecke, Helios University Hospital Wuppertal, 42283Wuppertal, Germany.",
    "Department of General, Visceral, and Transplant Surgery, Heidelberg University, Heidelberg University Hospital, 69120Heidelberg, Germany.",
    "Department of Neuroscience, Baylor College of Medicine, Houston, Texas77030, United States.",
    "Department of Pathology, The University of Texas Medical Branch, Galveston, Texas77555, United States."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 17,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Paul A Spezza"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Kshipra S Kapoor",
      "orcid": "0009-0007-9079-7373"
    },
    {
      "affs": [
        0,
        3
      ],
      "name": "Beatrice P Pforr"
    },
    {
      "affs": [
        0
      ],
      "name": "Xin Luo",
      "orcid": "0009-0006-3100-3847"
    },
    {
      "affs": [
        0
      ],
      "name": "Kaira A Church"
    },
    {
      "affs": [
        2,
        4
      ],
      "name": "Martin M Bell"
    },
    {
      "affs": [
        2
      ],
      "name": "Bo Fan"
    },
    {
      "affs": [
        0
      ],
      "name": "Seoyun Kong",
      "orcid": "0000-0001-6173-388X"
    },
    {
      "affs": [
        0
      ],
      "name": "Elena V Ramirez"
    },
    {
      "affs": [
        5
      ],
      "name": "Yi-Lin Chen"
    },
    {
      "affs": [
        0
      ],
      "name": "Fernanda G Kugeratski"
    },
    {
      "affs": [
        5
      ],
      "name": "Sibani Lisa Biswal",
      "orcid": "0000-0002-0610-835X"
    },
    {
      "affs": [
        0
      ],
      "name": "Kathleen M McAndrews",
      "orcid": "0000-0002-4092-4991"
    },
    {
      "affs": [
        6
      ],
      "name": "Florian Gebauer"
    },
    {
      "affs": [
        7
      ],
      "name": "Christoph Kahlert"
    },
    {
      "affs": [
        1,
        2,
        4,
        8
      ],
      "name": "Jacob T Robinson",
      "orcid": "0000-0002-3509-3054"
    },
    {
      "affs": [
        0,
        1,
        2,
        9
      ],
      "name": "Raghu Kalluri",
      "orcid": "0000-0002-2190-547X"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/2xfkt20936r3x.bib",
  "cited_by": 0,
  "doi": "10.1021/acsnano.6c08263",
  "fulltext_url": "https://europepmc.org/articles/PMC13585180",
  "id": "2xfkt20936r3x",
  "issns": [
    "1936-0851",
    "1936-086X"
  ],
  "landing_url": "https://europepmc.org/article/MED/42915039",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Extracellular vesicles in disease"
    ]
  ],
  "openalex": "W7207772878",
  "pdf_url": "https://europepmc.org/articles/PMC13585180?pdf=render",
  "pmcid": "PMC13585180",
  "pmid": "42915039",
  "published": "2026-09-01",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/2xfkt20936r3x.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:21:02Z",
      "native_id": "MED:42915039",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42915039"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Extracellular vesicles in disease",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Oncogenic KRAS Regulates Secretion of Extracellular Vesicles and Surface Membrane Charge via Regulation of Phosphatidylserine in Pancreatic Cancer.",
  "title_text": "Oncogenic KRAS Regulates Secretion of Extracellular Vesicles and Surface Membrane Charge via Regulation of Phosphatidylserine in Pancreatic Cancer.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:21:02Z",
  "url": "https://research.draconitic.com/paper/2xfkt20936r3x",
  "venue": "ACS nano",
  "versions": []
}