{
  "abstract": "Spring viremia of carp virus (SVCV) severely threatens cyprinid aquaculture, yet the role of autophagy in SVCV infection remains unclear. Here, SVCV infection was associated with enhanced autophagic activity and autophagic flux in common carp and EPC cells. Pharmacological perturbation of autophagy revealed that rapamycin treatment increased SVCV gene expression and aggravated SVCV-induced cytopathic effects, whereas chloroquine blocked autophagic flux, suppressed viral gene expression, and mitigated virus-induced cellular damage. Transcriptomic analysis identified 1,868 differentially expressed genes after chloroquine treatment, with major changes in lysosome/autophagy-related pathways, glycan degradation, ion transport, cell cycle, DNA replication, and DNA repair. Metabolomic and integrated multi-omics analyses revealed changes in amino sugar and nucleotide sugar metabolism, steroid biosynthesis, riboflavin metabolism, and amino acid metabolism. Functional validation showed that diflorasone and all-trans-retinal reduced SVCV-G expression, alleviated cytopathic effects, and improved survival after SVCV infection. These findings suggest that SVCV infection is associated with enhanced autophagic flux, whereas chloroquine treatment blocks autophagic flux and is accompanied by reduced SVCV gene expression.",
  "abstract_shown": true,
  "affiliations": [
    "College of Animal Science and Technology, Yangzhou University",
    "State Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Chinese Academy of Sciences",
    "University of Chinese Academy of Sciences",
    "Innovative Academy of Seed Design, Chinese Academy of Sciences",
    "Yizheng Water World Agricultural Ecological Park Co., Ltd"
  ],
  "article_type": "research support, non u.s. gov't",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Yuchao Zhu"
    },
    {
      "affs": [
        0
      ],
      "name": "Shuyang Chen"
    },
    {
      "affs": [
        0
      ],
      "name": "Yue Wang"
    },
    {
      "affs": [
        0
      ],
      "name": "Jian Shen"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Libo He"
    },
    {
      "affs": [
        1,
        3
      ],
      "name": "Yaping Wang"
    },
    {
      "affs": [
        4
      ],
      "name": "Dong Wang"
    },
    {
      "affs": [
        0
      ],
      "name": "Shuyan Miao"
    },
    {
      "affs": [
        0
      ],
      "name": "Guobin Chang"
    },
    {
      "affs": [
        0
      ],
      "name": "Pengfei Chu"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/2xgvqmdwj7p6b.bib",
  "cited_by": 0,
  "doi": "10.3389/fimmu.2026.1920055",
  "fulltext_url": "https://europepmc.org/articles/PMC13683750",
  "id": "2xgvqmdwj7p6b",
  "issns": [
    "1664-3224"
  ],
  "landing_url": "https://www.frontiersin.org/articles/10.3389/fimmu.2026.1920055/full",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2403|Aquaculture disease management and microbiota"
    ]
  ],
  "openalex": "W7220863274",
  "pdf_url": "https://europepmc.org/articles/PMC13683750?pdf=render",
  "pmcid": "PMC13683750",
  "pmid": "42909045",
  "published": "2026-10-07",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/2xgvqmdwj7p6b.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:15Z",
      "native_id": "10.3389/fimmu.2026.1920055",
      "source": "crossref",
      "url": "https://www.frontiersin.org/articles/10.3389/fimmu.2026.1920055/full"
    },
    {
      "first_seen": "2026-10-30T12:20:10Z",
      "native_id": "MED:42909045",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42909045"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:2403|Aquaculture disease management and microbiota",
      "subfield": "immunology"
    }
  ],
  "title": "Chloroquine inhibits Spring viremia of carp virus replication by modulating autophagy and metabolic pathways",
  "title_text": "Chloroquine inhibits Spring viremia of carp virus replication by modulating autophagy and metabolic pathways",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:20:10Z",
  "url": "https://research.draconitic.com/paper/2xgvqmdwj7p6b",
  "venue": "Frontiers in Immunology",
  "versions": []
}