{
  "abstract": "Background: Prostate cancer (PCa), particularly metastatic castration-resistant PCa (mCRPC), remains therapeutically challenging. Cuproptosis, a copper-dependent cell death driven by lipoylated tricarboxylic acid cycle proteins, is implicated in PCa progression, but whether ceruloplasmin (CP), a key copper-binding glycoprotein, modulates cuproptosis in PCa and the underlying mediators remain unknown.\n\nMethods: We integrated bioinformatic analysis of TCGA-PRAD data (550 PCa, 52 normal tissues) with in vitro validation in two androgen-independent CRPC cell lines: PC-3 cells received exogenous CP gradients, while 22Rv1 cells underwent CP knockdown and rescue experiments with qPCR, Western blot, MitoSOX flow cytometry, and CCK-8 assays.\n\nResults: Eighty CP-cuproptosis intersection genes were identified. Cuproptosis core genes (FDX1, DLST, LIAS, DLD) were significantly upregulated in PCa (all p<0.001), while CP showed no differential expression but weak positive correlations with these genes (r=0.205-0.300). Exogenous CP concentration-dependently reduced PC-3 metabolic activity and induced apoptosis-related morphology. In 22Rv1 cells, CP knockdown downregulated lncRNA STPG3-AS1, upregulated cuproptosis-related markers (FDX1, DLAT, lipoylated proteins) and mitochondrial ROS, and inhibited proliferation; STPG3-AS1 overexpression reversed all these effects. No gene showed independent prognostic value.\n\nConclusion: STPG3-AS1 mediates CP-regulated lipoylation and cuproptosis-associated molecular changes in CRPC cells, identifying a novel CP-STPG3-AS1-cuproptosis regulatory axis with therapeutic potential for advanced prostate cancer.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Urology, The Affiliated Dongguan Songshan Lake Central Hospital, Guangdong Medical University",
    "Department of Orthopedics, Dongguan Eighth People’s Hospital",
    "Department of Rehabilitation Medicine, The Affiliated Dongguan Songshan Lake Central Hospital, Guangdong Medical University"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 3,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Jia Wei He"
    },
    {
      "affs": [
        1
      ],
      "name": "Pei Zhen Li"
    },
    {
      "affs": [
        2
      ],
      "name": "Zhen Zhao Zhang"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/2xx9vkyewtnhp.bib",
  "cited_by": 0,
  "doi": "10.3389/fonc.2026.1989277",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13735617/",
  "id": "2xx9vkyewtnhp",
  "issns": [
    "2234-943X"
  ],
  "landing_url": "https://www.frontiersin.org/articles/10.3389/fonc.2026.1989277/full",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Original Research"
    ],
    [
      "openalex",
      "2740|Prostate Cancer Treatment and Research"
    ]
  ],
  "openalex": "W7223168693",
  "pdf_url": "https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2026.1989277/pdf",
  "pmcid": "PMC13735617",
  "pmid": "42991319",
  "published": "2026-10-22",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/2xx9vkyewtnhp.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:20:13Z",
      "native_id": "10.3389/fonc.2026.1989277",
      "source": "crossref",
      "url": "https://www.frontiersin.org/articles/10.3389/fonc.2026.1989277/full"
    },
    {
      "first_seen": "2026-11-09T12:21:16Z",
      "native_id": "PMC13735617",
      "source": "pmc",
      "url": "https://doi.org/10.3389/fonc.2026.1989277"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "issn:2234-943X",
      "subfield": "oncology"
    }
  ],
  "title": "The CP-STPG3-AS1 axis regulates lipoylation and cuproptosis in castration-resistant prostate cancer",
  "title_text": "The CP-STPG3-AS1 axis regulates lipoylation and cuproptosis in castration-resistant prostate cancer",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T12:21:16Z",
  "url": "https://research.draconitic.com/paper/2xx9vkyewtnhp",
  "venue": "Frontiers in Oncology",
  "versions": []
}