{
  "abstract": "Background: Here, we report the identification and comprehensive whole-genome characterization of a New Delhi metallo-β-lactamase 5 (blaNDM-5)-producing multidrug-resistant (MDR) monophasic Salmonella Typhimurium ST34 strain SYA001 from an immunocompromised rectal cancer patient. Monophasic Salmonella Typhimurium (Salmonella enterica subsp. enterica serovar 1,4,[5],12:i:-) is one of the leading causative agents of foodborne gastroenteritis and hospitalization worldwide, and the rapid emergence and spread of carbapenem-resistant strains have severely compromised clinical treatment and posed a major threat to public health.\n\nMethods: In this study, a series of standardized phenotypic assays were performed to characterize the microbiological, biochemical, and antimicrobial resistance profiles of strain SYA001. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), biochemical identification, and slide agglutination serotyping were used for species and serotype confirmation. Antimicrobial susceptibility testing was conducted using the VITEK 2 compact system, and six types of carbapenemases were screened via a colloidal gold immunochromatographic assay. Whole-genome sequencing (WGS) was performed on the DNBSEQ-T7 platform, and a comprehensive bioinformatics pipeline was applied to dissect the genomic structure, phylogenetic characteristics, antibiotic resistance determinants, plasmid profiles, prophage regions, Salmonella pathogenicity islands (SPIs), and protein-protein interaction (PPI) network of the strain.\n\nResults: Our results confirmed that SYA001 is a serovar 1,4,[5],12:i:- monophasic Salmonella Typhimurium belonging to the globally epidemic ST34 clone. The strain exhibited an MDR phenotype, with resistance to 7 classes of antibiotics including carbapenems, cephalosporins, and aminoglycosides, and was only susceptible to aztreonam, amikacin, tigecycline, and colistin. Genomic analysis identified the core carbapenem resistance gene blaNDM-5, along with multiple coexisting resistance genes including blaOXA-10, qnrS1, tet(A), sul2, and sul3 and so on. Four plasmid replicons (IncHI2, IncHI2A, IncQ1, p0111), 15 prophage regions, and a complete set of virulence-related SPIs (SPI-1 to SPI-5,SPI-9, SPI-13, SPI-14) were detected. PPI network analysis revealed AcrA as the core hub of the efflux-mediated resistance regulatory network.\n\nConclusion: This study provides genomic insights into blaNDM-5-producing MDR monophasic Salmonella. Typhimurium from an immunocompromised rectal cancer patient, supplements clinical surveillance data of carbapenem-resistant Salmonella in Hainan.",
  "abstract_shown": true,
  "affiliations": [
    "Clinical & Central Laboratory of Sanya People’s Hospital , , ,",
    "Department of Hematology and Oncology of Sanya People’s Hospital , , ,"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Shan Zhong"
    },
    {
      "affs": [
        1
      ],
      "name": "Lei Xing"
    },
    {
      "affs": [
        0
      ],
      "name": "Licheng Wang"
    },
    {
      "affs": [
        0
      ],
      "name": "Chongzhen Wang"
    },
    {
      "affs": [
        0
      ],
      "name": "Xiaoying Fu"
    },
    {
      "affs": [
        0
      ],
      "name": "Lixia Xu"
    },
    {
      "affs": [
        0
      ],
      "name": "Yuanli Li"
    },
    {
      "affs": [
        0
      ],
      "name": "Huaxiong Zhu"
    },
    {
      "affs": [
        0
      ],
      "name": "Lvfen He"
    },
    {
      "affs": [
        0
      ],
      "name": "Xiong Zhu"
    },
    {
      "affs": [
        0
      ],
      "name": "Huan Li"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/2yb8y41tbasz5.bib",
  "cited_by": 0,
  "doi": "10.3389/fcimb.2026.1942157",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13719422/?tool=EBI",
  "id": "2yb8y41tbasz5",
  "issns": [
    "2235-2988"
  ],
  "landing_url": "https://europepmc.org/article/PMC/PMC13719422",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Original Research"
    ],
    [
      "openalex",
      "1106|Salmonella and Campylobacter epidemiology"
    ]
  ],
  "openalex": "W7222455711",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13719422/pdf/?tool=EBI",
  "pmcid": "PMC13719422",
  "pmid": "42964160",
  "published": "2026-10-16",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/2yb8y41tbasz5.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T12:21:36Z",
      "native_id": "PMC:PMC13719422",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13719422"
    },
    {
      "first_seen": "2026-11-02T12:23:23Z",
      "native_id": "PMC13719422",
      "source": "pmc",
      "url": "https://doi.org/10.3389/fcimb.2026.1942157"
    }
  ],
  "subjects": [],
  "title": "Multidrug-resistant monophasic Salmonella Typhimurium ST34 strain producing blaNDM-5 carbapenemase isolated from a patient with rectal cancer in Hainan, China: comprehensive whole genome dissection of antibiotic resistance and virulence determinants",
  "title_text": "Multidrug-resistant monophasic Salmonella Typhimurium ST34 strain producing blaNDM-5 carbapenemase isolated from a patient with rectal cancer in Hainan, China: comprehensive whole genome dissection of antibiotic resistance and virulence determinants",
  "type": "peer-reviewed",
  "updated_at": "2026-11-03T12:20:29Z",
  "url": "https://research.draconitic.com/paper/2yb8y41tbasz5",
  "venue": "Frontiers in cellular and infection microbiology",
  "versions": []
}