{
  "abstract": "Animals perceive their immediate environment through their sensory systems. While these systems rely on well-defined neuronal circuits and receptor proteins encoded by the genome, they can also be modulated by internal physiological conditions or exogenous factors. We have previously shown that the presence of certain pathogenic bacteria in the larval gut can alter the specificity of bacterial peptidoglycan recognition in the adults that emerge from these larvae. However, the nature of the bacterial signal and the host cells and molecules involved in receiving and transducing this signal remained unknown. Our results identify uracil as the bacterial metabolite responsible for this priming and demonstrate that the effect is mediated by Duox-dependent production of reactive oxygen species (ROS) in the larval gut. We further show that specific expression of TrpA1 isoforms in a Gr66a-expressing neuron of the larval terminal organ is required for the adult response. Together, these findings reveal uracil as the bacterial cue and the Duo/ROS signaling and TrpA1/Gr66a modules as key mediators linking larval gut microbial signals and host integration to subsequent sensory system modulation in the adult.\n\nPathogenic bacteria in the larval fly gut can ‘prime’ the adult gustatory system to recognize bacterial peptidoglycans, but how? This study identifies Uracil as the bacteria-derived cue and characterizes a larval sensory pathway required for priming the adult.",
  "abstract_shown": true,
  "affiliations": [
    "Aix-Marseille Université, CNRS, IBDM, Marseille, France.",
    "Université Bourgogne Europe, Institut Agro, CNRS, INRAE, UMR CSGA, Dijon, France."
  ],
  "article_type": "Research Article",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
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        0
      ],
      "name": "Romane Milleville",
      "orcid": "0009-0006-9290-5027"
    },
    {
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        1
      ],
      "name": "Gérard Manière",
      "orcid": "0000-0003-1308-7291"
    },
    {
      "affs": [
        1
      ],
      "name": "Martine Berthelot-Grosjean",
      "orcid": "0000-0002-7168-2622"
    },
    {
      "affs": [
        1
      ],
      "name": "Olivier Lamotte"
    },
    {
      "affs": [
        1
      ],
      "name": "Yaël Grosjean",
      "orcid": "0000-0003-0689-8344"
    },
    {
      "affs": [
        0
      ],
      "name": "Julien Royet",
      "orcid": "0000-0002-5671-4833"
    },
    {
      "affs": [
        0
      ],
      "name": "Cyril Léopold Kurz",
      "orcid": "0000-0001-7081-3208"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/2z90xf3afw7c6.bib",
  "cited_by": 0,
  "doi": "10.1371/journal.pbio.3004042",
  "fulltext_url": "https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3004042",
  "id": "2z90xf3afw7c6",
  "issns": [
    "1545-7885",
    "1544-9173"
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  "landing_url": "https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3004042",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
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      "openalex",
      "2804|Neurobiology and Insect Physiology Research"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Nucleotides/Nucleobases/Uracils"
    ],
    [
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      "/Biology and life sciences/Cell biology/Cellular types/Animal cells/Neurons"
    ],
    [
      "plos",
      "/Biology and life sciences/Cell biology/Signal transduction/Cell signaling/Redox signaling"
    ],
    [
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      "/Biology and life sciences/Developmental biology/Life cycles/Larvae"
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    ],
    [
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      "/Biology and life sciences/Neuroscience/Cognitive science/Cognitive psychology/Perception/Sensory perception"
    ],
    [
      "plos",
      "/Biology and life sciences/Neuroscience/Neuroimaging/Calcium imaging"
    ],
    [
      "plos",
      "/Biology and life sciences/Neuroscience/Sensory perception"
    ],
    [
      "plos",
      "/Biology and life sciences/Physiology/Reproductive physiology/Eggs"
    ],
    [
      "plos",
      "/Biology and life sciences/Psychology/Cognitive psychology/Perception/Sensory perception"
    ],
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      "plos",
      "/Physical sciences/Chemistry/Chemical compounds/Organic compounds/Carbohydrates/Disaccharides/Sucrose"
    ],
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      "plos",
      "/Physical sciences/Chemistry/Organic chemistry/Organic compounds/Carbohydrates/Disaccharides/Sucrose"
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      "/Research and analysis methods/Imaging techniques/Neuroimaging/Calcium imaging"
    ],
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      "plos",
      "/Social sciences/Psychology/Cognitive psychology/Perception/Sensory perception"
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  "pdf_url": "https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.3004042&type=printable",
  "pmcid": "PMC13657326",
  "pmid": "42832584",
  "published": "2026-10-05",
  "publisher": "Public Library of Science",
  "ris": "https://research.draconitic.com/paper/2z90xf3afw7c6.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:37Z",
      "native_id": "10.1371/journal.pbio.3004042",
      "source": "plos",
      "url": "https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3004042"
    },
    {
      "first_seen": "2026-10-30T12:17:50Z",
      "native_id": "hal-05783149",
      "source": "hal",
      "url": "https://hal.science/hal-05783149v1"
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  "subjects": [
    {
      "field": "biology",
      "origin": "plos:cell biology",
      "subfield": "cell-biology"
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    {
      "field": "biology",
      "origin": "plos:neuroscience",
      "subfield": "neuroscience"
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  ],
  "title": "Uracil-driven ROS signaling and larval TrpA1-B neuron activation are required for priming the adult Drosophila gustatory response to bacteria",
  "title_text": "Uracil-driven ROS signaling and larval TrpA1-B neuron activation are required for priming the adult Drosophila gustatory response to bacteria",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:10Z",
  "url": "https://research.draconitic.com/paper/2z90xf3afw7c6",
  "venue": "PLOS Biology",
  "versions": []
}