{
  "abstract": "Methane is a powerful greenhouse gas. Typically, a large fraction of methane formed in coastal sediments is removed via anaerobic oxidation of methane (AOM). Here, we demonstrate the potential for a range of AOM pathways in brackish coastal sediments by ANME-2a archaea. At our study site, geochemical profiles indicate that AOM is primarily restricted to a shallow, metal oxide-rich sulfate-methane transition zone (SMTZ). ANME-2a were the sole methanotrophs detected, and metatranscriptomics showed the highest expression levels of the ANME-2a genes in the SMTZ. AOM activity was observed in sediment incubations with various electron acceptors, including sulfate, metal oxides, and the organic matter analog graphene oxide. Highest potential rates were observed in sediments from below the SMTZ, pointing toward fast stimulation of the deeper methanotrophic community when alleviating the electron acceptor limitation. The variety of AOM pathways and persistence of methanotrophs below the SMTZ likely contribute to the resilience of the microbial methane filter in brackish coastal sediments.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Microbiology, Radboud Institute for Biological and Environmental Sciences, Radboud University, Heyendaalseweg 135, 6525AJ Nijmegen, Netherlands.",
    "Department of Earth Sciences, Utrecht University, Princetonlaan 8a, 3584 CB Utrecht, Netherlands.",
    "Stockholm University Baltic Sea Center, Svante Arrheniusväg 20F, Stockholm, Sweden.",
    "Institute for Marine and Atmospheric Research Utrecht, Utrecht University, Princetonplein 5, 3584CC Utrecht, Netherlands."
  ],
  "article_type": "Research Article",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Robin Klomp",
      "orcid": "0009-0008-3588-6792"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Anna J. Wallenius",
      "orcid": "0000-0002-1510-4318"
    },
    {
      "affs": [
        0
      ],
      "name": "Merijn A. W. Schutgens",
      "orcid": "0009-0000-1227-6539"
    },
    {
      "affs": [
        0
      ],
      "name": "Theo van Alen",
      "orcid": "0009-0008-5602-3937"
    },
    {
      "affs": [
        3
      ],
      "name": "Thomas Röckmann",
      "orcid": "0000-0002-6688-8968"
    },
    {
      "affs": [
        0
      ],
      "name": "Mike S. M. Jetten",
      "orcid": "0000-0002-4691-7039"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Caroline P. Slomp",
      "orcid": "0000-0002-7272-0109"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/307bafe86mb5q.bib",
  "cited_by": 0,
  "doi": "10.1126/sciadv.aei5868",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13721985/?tool=EBI",
  "id": "307bafe86mb5q",
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    "2375-2548"
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  "landing_url": "https://www.science.org/doi/abs/10.1126/sciadv.aei5868",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
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  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
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  "native_subjects": [
    [
      "heading",
      "Earth, Environmental, Ecological, and Space Sciences"
    ],
    [
      "heading",
      "Geochemistry"
    ],
    [
      "heading",
      "Microbiology"
    ],
    [
      "heading",
      "Research Article"
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      "heading",
      "SciAdv r-articles"
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  "openalex": null,
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13721985/pdf/?tool=EBI",
  "pmcid": "PMC13721985",
  "pmid": "42962030",
  "published": "2026-10-30",
  "publisher": "American Association for the Advancement of Science (AAAS)",
  "ris": "https://research.draconitic.com/paper/307bafe86mb5q.ris",
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  "subjects": [
    {
      "field": "biology",
      "origin": "heading:Microbiology",
      "subfield": "microbiology"
    },
    {
      "field": "earth",
      "origin": "heading:Geochemistry",
      "subfield": "geology-geochemistry"
    }
  ],
  "title": "ANME-2a perform methane oxidation in brackish coastal sediments via multiple pathways",
  "title_text": "ANME-2a perform methane oxidation in brackish coastal sediments via multiple pathways",
  "type": "peer-reviewed",
  "updated_at": "2026-11-03T12:22:34Z",
  "url": "https://research.draconitic.com/paper/307bafe86mb5q",
  "venue": "Science Advances",
  "versions": []
}