{
  "abstract": "The detection rate of avian leukosis virus subgroup K (ALV-K) has been increasing annually in Chinese local breeds, its poor replicative capacity limits the success of virus isolation. To improve ALV-K eradication, this study assessed the detection limits of the magnetic-particle chemiluminescent assay and ELISA kits using a standardized virus stock. Additionally, we also determined the impact of serial passage during virus isolation on the positive detection rate. Using a standardized ALV-K stock, the magnetic-particle chemiluminescent kit detected positive signals at a 256-fold dilution, whereas the most sensitive ELISA kit reached its limit at a 64-fold dilution. When applied to 13 plasma samples from a local chicken flock, the magnetic-particle chemiluminescent kit at passage 0 yielded a detection rate of 76.9% (10/13), compared to only 15.4% (2/13) by ELISA. After a single cell passage, the chemiluminescent method achieved 100% positivity (13/13), while ELISA required two passages to reach 61.5% (8/13). Quantitative real-time PCR (qPCR) confirmed the presence of ALV-K in at least 9 plasma samples, corroborating the chemiluminescent results. These results demonstrate that the magnetic-particle chemiluminescent assay exhibits significantly higher sensitivity than ELISA and that cell passaging markedly enhances the positive rate of virus isolation. By integrating a highly sensitive chemiluminescent assay with an optimized viral isolation protocol, this study provides a novel strategy for facilitating ALV-K eradication in local Chinese chicken flocks.",
  "abstract_shown": true,
  "affiliations": [
    "College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an, 271018, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Tai'an, 271018, China.",
    "College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an, 271018, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Tai'an, 271018, China. Electronic address: zhren92@163.com.",
    "College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an, 271018, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Tai'an, 271018, China. Electronic address: zhaopeng@sdau.edu.cn."
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Ruiqiang Geng"
    },
    {
      "affs": [
        0
      ],
      "name": "Mengyu Song"
    },
    {
      "affs": [
        0
      ],
      "name": "Wenping Cui"
    },
    {
      "affs": [
        0
      ],
      "name": "Yixin Wang"
    },
    {
      "affs": [
        0
      ],
      "name": "Shuang Chang"
    },
    {
      "affs": [
        1
      ],
      "name": "Zhihao Ren"
    },
    {
      "affs": [
        2
      ],
      "name": "Peng Zhao"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/30bgdr0fdmqf9.bib",
  "cited_by": 0,
  "doi": "10.1016/j.psj.2026.107577",
  "fulltext_url": "https://europepmc.org/articles/PMC13691134",
  "id": "30bgdr0fdmqf9",
  "issns": [
    "0032-5791",
    "1525-3171"
  ],
  "landing_url": "https://europepmc.org/article/MED/42896422",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1103|Animal Virus Infections Studies"
    ]
  ],
  "openalex": "W7172511327",
  "pdf_url": "https://europepmc.org/articles/PMC13691134?pdf=render",
  "pmcid": "PMC13691134",
  "pmid": "42896422",
  "published": "2026-08-05",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/30bgdr0fdmqf9.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:41Z",
      "native_id": "MED:42896422",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42896422"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1103|Animal Virus Infections Studies",
      "subfield": "zoology-behaviour"
    }
  ],
  "title": "Research note: Improved virus isolation strategies enhance the detection rate for avian leukosis virus eradication in a local chicken breed in China.",
  "title_text": "Research note: Improved virus isolation strategies enhance the detection rate for avian leukosis virus eradication in a local chicken breed in China.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:41Z",
  "url": "https://research.draconitic.com/paper/30bgdr0fdmqf9",
  "venue": "Poultry science",
  "versions": []
}