{
  "abstract": "Since the development of CRISPR-based genome editing tools, a number of novel technologies have emerged. This includes Prime-Editing that acts as a search and replace genome editing tool. Prime-Editing has been deployed across multiple clades, including in a few flowering plants. Here, we report on the development of an efficient Prime Editor (PE) for the model bryophyte Marchantia. Initial tests were conducted on Acetolactate Synthase as a target and revealed an average efficiency above 40%. The system has been developed in the GoldenGate cloning system, facilitating construct design. The development of PE in Marchantia expands the Genome-Editing tools available for this emerging model in plant biology.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "name": "Benoit Danilo"
    },
    {
      "name": "Aurélie Quillien"
    },
    {
      "name": "Cristina Rojas-Latorre"
    },
    {
      "name": "Zaïd Nibani"
    },
    {
      "name": "Clara Mestre"
    },
    {
      "name": "Pierre-Marc Delaux"
    },
    {
      "name": "Dominique Lauressergues"
    },
    {
      "name": "Julie Neveu"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/31h4z5xgvyp23.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.08.07.743462",
  "fulltext_url": null,
  "id": "31h4z5xgvyp23",
  "issns": [],
  "landing_url": "https://hal.science/hal-05782767v1",
  "language": "en",
  "licence": "CC-BY-NC",
  "licence_label": "CC BY-NC",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "openalex",
      "1312|CRISPR and Genetic Engineering"
    ]
  ],
  "openalex": "W7202203617",
  "pdf_url": "https://www.biorxiv.org/content/biorxiv/early/2026/08/11/2026.08.07.743462.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-08-11",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/31h4z5xgvyp23.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:50Z",
      "native_id": "hal-05782767",
      "source": "hal",
      "url": "https://hal.science/hal-05782767v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|CRISPR and Genetic Engineering",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Prime-Editing in Marchantia paleacea : Expanding the Genome-Editing Toolbox in Bryophytes",
  "title_text": "Prime-Editing in Marchantia paleacea : Expanding the Genome-Editing Toolbox in Bryophytes",
  "type": "preprint",
  "updated_at": "2026-10-30T12:17:50Z",
  "url": "https://research.draconitic.com/paper/31h4z5xgvyp23",
  "venue": "HAL",
  "versions": []
}