{
  "abstract": "Mitochondria are essential organelles containing their own genomes, encoding a few proteins essential for energy production. Most of the mitochondrial proteins are nucleus-encoded, translated as precursors in the cytosol, with a large fraction of these precursors properly addressed by an N-terminal mitochondrial targeting sequence (MTS). These MTS share common features but no consensus sequence can explain their functionality nor the precursors-specific determinants of mitochondrial import. To help understand this mechanism, we created a simple computational model to generate functional synthetic MTS while maintaining a tight control on the design parameters. Using the budding yeast, we demonstrated the presence of precursors-specific import capabilities in addressing artificially nucleus-relocated OXPHOS proteins. Our research confirms the existence of cargo-dependent functional compatibilities and takes us one step closer towards improving gene therapy-based treatment of mitochondrial diseases.",
  "abstract_shown": true,
  "affiliations": [
    "Manchester Institute of Biotechnology, University of Manchester , 131 Princess Street, M1 7DN, Manchester ,",
    "CNRS, IBGC , UMR 5095, University of Bordeaux, F-33000 Bordeaux ,"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Kewin Gombeau",
      "orcid": "0000-0003-3062-6365"
    },
    {
      "affs": [
        0
      ],
      "name": "Raymond Wan",
      "orcid": "0000-0003-3202-7008"
    },
    {
      "affs": [
        0
      ],
      "name": "Joshua S James",
      "orcid": "0000-0003-2058-4210"
    },
    {
      "affs": [
        1
      ],
      "name": "Déborah Tribouillard-Tanvier",
      "orcid": "0000-0002-2290-5375"
    },
    {
      "affs": [
        0
      ],
      "name": "Yizhi Cai",
      "orcid": "0000-0003-1663-2865"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/31k99481nrm4s.bib",
  "cited_by": 0,
  "doi": "10.1093/nar/gkag986",
  "fulltext_url": "https://europepmc.org/articles/PMC13684279",
  "id": "31k99481nrm4s",
  "issns": [
    "0305-1048",
    "1362-4962"
  ],
  "landing_url": "https://academic.oup.com/nar/article/doi/10.1093/nar/gkag986/8959888",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Fungal and yeast genetics research"
    ]
  ],
  "openalex": "W7213913808",
  "pdf_url": "https://academic.oup.com/nar/article-pdf/54/19/gkag986/72218618/gkag986.pdf",
  "pmcid": "PMC13684279",
  "pmid": "42908398",
  "published": "2026-10-01",
  "publisher": "Oxford University Press (OUP)",
  "ris": "https://research.draconitic.com/paper/31k99481nrm4s.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:50Z",
      "native_id": "10.1093/nar/gkag986",
      "source": "crossref",
      "url": "https://academic.oup.com/nar/article/doi/10.1093/nar/gkag986/8959888"
    },
    {
      "first_seen": "2026-10-30T12:19:57Z",
      "native_id": "MED:42908398",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42908398"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Fungal and yeast genetics research",
      "subfield": "molecular-biology"
    }
  ],
  "title": "TargetMITO: a rule-based model for generating functional synthetic mitochondrial targeting sequences in yeast",
  "title_text": "TargetMITO: a rule-based model for generating functional synthetic mitochondrial targeting sequences in yeast",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:57Z",
  "url": "https://research.draconitic.com/paper/31k99481nrm4s",
  "venue": "Nucleic Acids Research",
  "versions": []
}