{
  "abstract": "To explore the protective effect of Breviscapine against myocardial ischemia-reperfusion (I/R) injury and its mechanism. The in vivo and in vitro models of myocardial I/R injury were first established and treated with different concentrations of Bre. The myocardial infarct size was measured, while histopathological changes of myocardial tissue were evaluated by hematoxylin-eosin (HE) staining, and cardiac function-related parameters were assessed via echocardiography. Prussian blue staining was performed to visualize ferroptosis; enzyme-linked immunosorbent assay (ELISA) was used to detect the expression levels of creatine kinase-MB (CK-MB) and lactate dehydrogenase (LDH); flow cytometry was employed to determine the apoptosis rate of cardiomyocytes. Western blot (WB) and qRT-PCR were conducted to examine the levels of ferroptosis-related molecules (NOX1, NRF2, GPX4 and SLC7A11), respectively. Meanwhile, oxidative stress-related indices associated with ferroptosis, such as malondialdehyde (MDA), superoxide dismutase (SOD) and reactive oxygen species (ROS), were determined. Finally, a specific NRF2 inhibitor was administered for intervention, and changes in the above indicators were observed. Bre dose-dependently alleviated myocardial histological damage, reduced infarct size, improved cardiac function (elevated EF/FS, decreased LVEDV/LVESV), and inhibited cardiomyocyte apoptosis. Bre also suppressed ferroptosis, as shown by reduced Fe²⁺accumulation, MDA, GSSG, ROS, and NOX1 expression, and enhanced SOD activity, GSH content, and NRF2/SLC7A11/GPX4 expression. NRF2 inhibitor ML385 reversed these effects. Bre alleviates myocardial I/R injury by inhibiting ferroptosis via activating the NRF2/SLC7A11/GPX4 pathway.",
  "abstract_shown": true,
  "affiliations": [
    "Division of Cardiac Care Unit, Department of Cardiology, Wuhan Asia Heart Hospital",
    "Department of Cardiology, Hubei Provincial Hospital of Traditional Chinese Medicine",
    "Hubei University of Chinese Medicine"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "An’an Tan"
    },
    {
      "affs": [
        1
      ],
      "name": "Yingzhe Xiong"
    },
    {
      "affs": [
        1
      ],
      "name": "Jiawei Wu"
    },
    {
      "affs": [
        1
      ],
      "name": "Qiangqiang Tian"
    },
    {
      "affs": [
        2
      ],
      "name": "Chen Chen"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/32cp4v3g1zjcj.bib",
  "cited_by": 0,
  "doi": "10.1038/s41598-026-72823-1",
  "fulltext_url": "https://doi.org/10.1038/s41598-026-72823-1",
  "id": "32cp4v3g1zjcj",
  "issns": [
    "2045-2322"
  ],
  "landing_url": "https://doi.org/10.1038/s41598-026-72823-1",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "lcc",
      "Q"
    ],
    [
      "lcc",
      "R"
    ],
    [
      "openalex",
      "2734|Cardiac Ischemia and Reperfusion"
    ]
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  "openalex": "W7214148867",
  "pdf_url": "https://www.nature.com/articles/s41598-026-72823-1_reference.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-01",
  "publisher": "Nature Portfolio",
  "ris": "https://research.draconitic.com/paper/32cp4v3g1zjcj.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:45:54Z",
      "native_id": "dc8fccefe2d7421690ed9ef282c5b1a9",
      "source": "doaj",
      "url": "https://doi.org/10.1038/s41598-026-72823-1"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2734|Cardiac Ischemia and Reperfusion",
      "subfield": "general"
    }
  ],
  "title": "Breviscapine attenuates myocardial ischemia-reperfusion injury by inhibiting ferroptosis via activation of NRF2/SLC7A11/GPX4 pathway",
  "title_text": "Breviscapine attenuates myocardial ischemia-reperfusion injury by inhibiting ferroptosis via activation of NRF2/SLC7A11/GPX4 pathway",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T13:45:54Z",
  "url": "https://research.draconitic.com/paper/32cp4v3g1zjcj",
  "venue": "Scientific Reports",
  "versions": []
}