{
  "abstract": "We present a protocol for establishing murine models of bisphosphonate-related osteonecrosis of the jaw (BRONJ) and osteoradionecrosis of the jaw (ORNJ). We describe steps for a uniform, low-trauma tooth extraction technique with disease-specific induction, systemic zoledronic acid for BRONJ and precision-targeted irradiation for ORNJ, to generate robust and clinically relevant models of jaw osteonecrosis, approaching minimizes procedural variability and off-target effects, yielding robust, clinically relevant phenotypes. This protocol provides a standard and minimally invasive framework for investigating jaw osteonecrosis arising from distinct etiologies.",
  "abstract_shown": true,
  "affiliations": [
    "State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China; Tissue and Tumor Microenvironments Lab, Cancer Discovery and Regenerative Medicine Program, Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore 636921, Singapore.",
    "State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.",
    "Tissue and Tumor Microenvironments Lab, Cancer Discovery and Regenerative Medicine Program, Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore 636921, Singapore.",
    "Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN 55905, USA; Robert and Arlene Kogod Center on Aging, Mayo Clinic, Rochester, MN 55905, USA; Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905, USA.",
    "Department of Orthopaedic Surgery, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA, USA; Graduate Program in Cell Biology and Regenerative Medicine, Thomas Jefferson University, Philadelphia, PA, USA.",
    "Tissue and Tumor Microenvironments Lab, Cancer Discovery and Regenerative Medicine Program, Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore 636921, Singapore; Multidisciplinary Institute of Ageing (MIA-Portugal), University of Coimbra, 3004-504 Coimbra, Portugal. Electronic address: anjali.pkusumbe@ntu.edu.sg."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Zhangfan Ding"
    },
    {
      "affs": [
        1
      ],
      "name": "Jiayuan Zhang"
    },
    {
      "affs": [
        2
      ],
      "name": "Hanyu Liu"
    },
    {
      "affs": [
        3
      ],
      "name": "Abhishek Chandra",
      "orcid": "0000-0001-9423-9669"
    },
    {
      "affs": [
        4
      ],
      "name": "Makarand V Risbud"
    },
    {
      "affs": [
        5
      ],
      "name": "Anjali P Kusumbe",
      "orcid": "0000-0002-1911-5495"
    },
    {
      "name": "Junyu Chen"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/32rw9mgaszcz5.bib",
  "cited_by": 0,
  "doi": "10.1016/j.xpro.2026.104851",
  "fulltext_url": "https://europepmc.org/articles/PMC13670947",
  "id": "32rw9mgaszcz5",
  "issns": [
    "2666-1667"
  ],
  "landing_url": "https://europepmc.org/article/MED/42832338",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2730|Bone health and treatments"
    ]
  ],
  "openalex": "W7220387033",
  "pdf_url": "https://europepmc.org/articles/PMC13670947?pdf=render",
  "pmcid": "PMC13670947",
  "pmid": "42832338",
  "published": "2026-10-05",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/32rw9mgaszcz5.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:10Z",
      "native_id": "MED:42832338",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42832338"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2730|Bone health and treatments",
      "subfield": "oncology"
    }
  ],
  "title": "Protocol for standardized minimally invasive mouse models of bisphosphonate-related and radiation-induced jaw osteonecrosis.",
  "title_text": "Protocol for standardized minimally invasive mouse models of bisphosphonate-related and radiation-induced jaw osteonecrosis.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:10Z",
  "url": "https://research.draconitic.com/paper/32rw9mgaszcz5",
  "venue": "STAR protocols",
  "versions": []
}