{
  "abstract": "Aim: LGALS3BP (also known as Galectin-3-binding protein, Gal-3BP, or 90 K) is a multifunctional secreted glycoprotein involved in tumor progression and has emerged as a potential biomarker and therapeutic target in several malignancies, including neuroblastoma (NB). Increasing evidence suggests that LGALS3BP contributes to shaping the NB tumor microenvironment (TME), particularly through its enrichment on tumor-derived extracellular vesicles (EVs), where it may regulate tumor-stroma interplay. We investigated whether LGALS3BP contributes to stromal cell modulation in NB and is associated with changes in EV cargo composition.\n\nMethods: A patient-derived NB cell line naturally lacking LGALS3BP (hNB CTRL) and a matched counterpart ectopically re-expressing LGALS3BP (hNB LGALS3BP) were used. Bone marrow-derived mesenchymal stem cells (BMMSCs) were exposed to NB-derived conditioned media (CM) to assess cytokine secretion and LGALS3BP detection in recipient cells. EV cargo was analyzed by microRNA (miRNA) and proteomic profiling. Selected candidates were validated by qPCR and immunoblotting on NB-derived EVs.\n\nResults: LGALS3BP-containing CM was associated with an altered BMMSCs secretory profile, while tumor-derived extracellular LGALS3BP was detected in BMMSCs. Seven miRNAs were enriched in LGALS3BP-EVs and reduced in donor cells; four were increased in BMMSCs following EV exposure. Proteomic analysis revealed enrichment of RNA-binding proteins, including SRSF1, in LGALS3BP-EVs.\n\nConclusions: LGALS3BP is associated with altered EV cargo composition and BMMSCs secretory responses, potentially contributing to tumor-stroma communication within the NB TME.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Innovative Technologies in Medicine and Dentistry, “G. d’Annunzio” University of Chieti-Pescara",
    "Center for Advanced Studies and Technology (CAST), “G. d’Annunzio” University of Chieti-Pescara",
    "Department of Neuroscience, Imaging and Clinical Sciences, School of Medicine and Health Sciences, “G. d’Annunzio” University of Chieti-Pescara",
    "Unit of Molecular Genetics, Center for Advanced Studies and Technology (CAST), “G. d’Annunzio” University of Chieti-Pescara",
    "Department of Science, “G. d’Annunzio” University of Chieti-Pescara"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 12,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Ilaria Cela"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Fani Konstantinidou"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Laura Amodei"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Arianna Mercatelli"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Francesca Piermatteo"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Sara Santoro"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Asia Pece"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Maria Clara Staropoli"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Alessia Lamolinara"
    },
    {
      "affs": [
        1,
        4
      ],
      "name": "Emily Capone"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Valentina Gatta"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Gianluca Sala"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/33q55ck7azgfq.bib",
  "cited_by": 0,
  "doi": "10.3389/fonc.2026.1966526",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13716570/?tool=EBI",
  "id": "33q55ck7azgfq",
  "issns": [
    "2234-943X"
  ],
  "landing_url": "https://www.frontiersin.org/articles/10.3389/fonc.2026.1966526/full",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Original Research"
    ],
    [
      "openalex",
      "1312|Extracellular vesicles in disease"
    ]
  ],
  "openalex": "W7222164564",
  "pdf_url": "https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2026.1966526/pdf",
  "pmcid": "PMC13716570",
  "pmid": "42959018",
  "published": "2026-10-15",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/33q55ck7azgfq.ris",
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  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Extracellular vesicles in disease",
      "subfield": "molecular-biology"
    }
  ],
  "title": "LGALS3BP is associated with selective cargo enrichment in neuroblastoma extracellular vesicles",
  "title_text": "LGALS3BP is associated with selective cargo enrichment in neuroblastoma extracellular vesicles",
  "type": "peer-reviewed",
  "updated_at": "2026-11-02T12:21:04Z",
  "url": "https://research.draconitic.com/paper/33q55ck7azgfq",
  "venue": "Frontiers in Oncology",
  "versions": []
}