{
  "abstract": "BackgroundPancreatic cancer (PCA) is a highly lethal malignancy with limited biomarkers for early diagnosis. Bile-derived epitranscriptomic biomarkers remain largely unexplored. We investigated whether bile-derived RNA N6-methyladenosine (m6A) methylation could serve as a diagnostic biomarker for PCA.MethodsBile samples were collected from patients with PCA (n = 7), biliary tract cancer (BTC; n = 33), and common bile duct (CBD) stones (n = 10). Pancreatic juice samples from patients with pancreatic neoplastic lesions (PNLs; n = 2) and PCA (n = 5) were analyzed as an exploratory cohort. Global RNA m6A was quantified in duplicate using an ELISA-based colorimetric assay. Diagnostic performance was evaluated using receiver operating characteristic (ROC) analysis.ResultsBile-derived RNA m6A levels were higher in PCA than in the comparison groups. Bile-derived RNA m6A discriminated PCA from the combined BTC and CBD stone groups with an AUC of 0.993 (bootstrap 95% CI, 0.970–1.000). The exploratory cut-off of 5.50% yielded 100.0% sensitivity and 97.7% specificity. In the exploratory pancreatic juice cohort, the AUC was 0.600 (bootstrap 95% CI, 0.200–1.000), with a 4.16% cut-off, 60.0% sensitivity, and 100.0% specificity.ConclusionBile-derived global RNA m6A methylation was associated with PCA in this proof-of-concept cohort. The diagnostic performance and data-derived cut-off are preliminary and require validation in larger prospective independent multicenter cohorts before clinical application.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": "Brief Research Report",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "name": "Jin-Yi Han"
    },
    {
      "name": "Keun Soo Ahn"
    },
    {
      "name": "Min Jae Kim"
    },
    {
      "name": "In Soo Cho"
    },
    {
      "name": "Tae-Seok Kim"
    },
    {
      "name": "Yong Hoon Kim"
    },
    {
      "name": "Kyung In Shin"
    },
    {
      "name": "Kwang Bum Cho"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/33r43efjx2azm.bib",
  "cited_by": 0,
  "doi": "10.3389/fonc.2026.1955611",
  "fulltext_url": null,
  "id": "33r43efjx2azm",
  "issns": [
    "2234-943X"
  ],
  "landing_url": "https://www.frontiersin.org/articles/10.3389/fonc.2026.1955611",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "feed-journal",
      "medicine/oncology"
    ]
  ],
  "openalex": null,
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-11-03",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/33r43efjx2azm.ris",
  "sources": [
    {
      "first_seen": "2026-11-03T06:20:19Z",
      "native_id": "10.3389/fonc.2026.1955611",
      "source": "feed-frontiers",
      "url": "https://www.frontiersin.org/articles/10.3389/fonc.2026.1955611"
    },
    {
      "first_seen": "2026-11-03T12:21:34Z",
      "native_id": "10.3389/fonc.2026.1955611",
      "source": "crossref",
      "url": "https://www.frontiersin.org/articles/10.3389/fonc.2026.1955611/full"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "feed-journal:medicine/oncology",
      "subfield": "oncology"
    }
  ],
  "title": "Bile-derived RNA N6-methyladenosine (m6A) methylation as a novel liquid biopsy biomarker for pancreatic cancer",
  "title_text": "Bile-derived RNA N6-methyladenosine (m6A) methylation as a novel liquid biopsy biomarker for pancreatic cancer",
  "type": "peer-reviewed",
  "updated_at": "2026-11-05T00:21:11Z",
  "url": "https://research.draconitic.com/paper/33r43efjx2azm",
  "venue": "Frontiers in Oncology",
  "versions": []
}