{
  "abstract": "Dirigent (DIR) proteins determine the regio- and stereoselectivity of phenoxy radical coupling between monolignols during (neo)lignan synthesis. However, the role of DIR proteins and lignans in plant development and stress responses remains to be clarified. Here, we show that ArabidopsisDIRIGENT13 (DIR13) is a direct target of cytokinin signaling and that its expression is strongly induced through direct binding of the cytokinin-responsive transcription factors ARR1, ARR10, and ARR12 to the DIR13 promoter. DIR13 is localized in the root endodermis and at the margins of lateral root primordia and emerging lateral roots. Although dispensable for Casparian strip formation, DIR13 promotes both primary and lateral root growth. Untargeted metabolomics and imaging mass spectrometry analyses revealed that DIR13 promotes (neo)lignan synthesis in primary and lateral roots. DIR13 attenuates the inhibitory effects of salt stress on germination and root growth. Moreover, DIR13 promotes reactive oxygen species (ROS) accumulation under both control and salt-stress conditions. Cytokinin further enhances salt-induced ROS production, probably through the synergistic activation of DIR13 transcription by salt and cytokinin. Our results suggest that lignans affect root growth, likely by modulating ROS homeostasis through their ROS-scavenging activity. Overall, we reveal a role for DIR13-produced lignans in ROS-regulated abiotic stress tolerance and the control of root architecture.",
  "abstract_shown": true,
  "affiliations": [
    "CEITEC - Central European Institute of Technology, Masaryk University, Brno, Czech Republic; National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Brno, Czech Republic",
    "Université Paris-Saclay, INRAE, AgroParisTech, Institut Jean-Pierre Bourgin (IJPB), 78000 Versailles, France",
    "CEITEC - Central European Institute of Technology, Masaryk University, Brno, Czech Republic",
    "Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan, China; Biotechnology Center in Southern Taiwan, Academia Sinica, Tainan, Taiwan, China",
    "Center for Plant Molecular Biology, University of Tübingen, Tübingen, Germany; Cluster of Excellence GreenRobust, University of Tübingen, Tübingen, Germany",
    "Organismal and Evolutionary Biology Research Programme, Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland",
    "CEITEC Brno University of Technology, Brno, Czech Republic",
    "Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia; Novosibirsk State University, Novosibirsk, Russia",
    "Institute of Plant Molecular Biology, Biology Centre, Czech Academy of Sciences, České Budějovice, Czech Republic; Faculty of Science, University of South Bohemia, 37005 České Budějovice, Czech Republic",
    "CEITEC - Central European Institute of Technology, Masaryk University, Brno, Czech Republic; National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Brno, Czech Republic. Electronic address: jan.hejatko@ceitec.muni.cz"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 20,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Alesia Melnikava",
      "orcid": "0000-0002-9298-3555"
    },
    {
      "affs": [
        1
      ],
      "name": "François Perreau",
      "orcid": "0000-0001-7873-9866"
    },
    {
      "affs": [
        2
      ],
      "name": "Candelas Paniagua",
      "orcid": "0000-0002-3263-1996"
    },
    {
      "affs": [
        3
      ],
      "name": "Sy‐Chyi Cheng",
      "orcid": "0000-0001-5017-0911"
    },
    {
      "affs": [
        3
      ],
      "name": "Li-Min Huang"
    },
    {
      "affs": [
        0
      ],
      "name": "Blanka Pekárová"
    },
    {
      "affs": [
        4
      ],
      "name": "Kenneth Wayne Berendzen",
      "orcid": "0000-0001-9870-6164"
    },
    {
      "affs": [
        4
      ],
      "name": "Virtudes Mira‐Rodado",
      "orcid": "0000-0003-0492-7885"
    },
    {
      "affs": [
        5
      ],
      "name": "Matteo Citterico",
      "orcid": "0000-0002-7585-4401"
    },
    {
      "affs": [
        6
      ],
      "name": "Anna Tvrdoňová"
    },
    {
      "affs": [
        7
      ],
      "name": "Elena V. Ubogoeva",
      "orcid": "0000-0002-8315-1191"
    },
    {
      "affs": [
        7
      ],
      "name": "Vladislav A. Dolgikh",
      "orcid": "0009-0009-2975-8329"
    },
    {
      "affs": [
        7
      ],
      "name": "Elena V. Zemlyanskaya",
      "orcid": "0009-0005-7316-7690"
    },
    {
      "affs": [
        6
      ],
      "name": "Eric Daniel Głowacki",
      "orcid": "0000-0002-0280-8017"
    },
    {
      "affs": [
        8
      ],
      "name": "Michael Wrzaczek",
      "orcid": "0000-0002-5946-9060"
    },
    {
      "affs": [
        4
      ],
      "name": "Klaus Harter",
      "orcid": "0000-0002-2150-6970"
    },
    {
      "affs": [
        3
      ],
      "name": "Yuliang Yang",
      "orcid": "0000-0002-3533-5148"
    },
    {
      "affs": [
        1
      ],
      "name": "Grégory Mouille",
      "orcid": "0000-0002-5493-754X"
    },
    {
      "affs": [
        2
      ],
      "name": "Dominique Arnaud",
      "orcid": "0000-0003-4898-1656"
    },
    {
      "affs": [
        9
      ],
      "name": "Jan Hejátko",
      "orcid": "0000-0002-2622-6046"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/33r8pq4fv6vax.bib",
  "cited_by": 6,
  "doi": "10.1016/j.xplc.2026.101972",
  "fulltext_url": null,
  "id": "33r8pq4fv6vax",
  "issns": [
    "2590-3462"
  ],
  "landing_url": "https://doi.org/10.1016/j.xplc.2026.101972",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1110|Plant Molecular Biology Research"
    ]
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  "openalex": "W7165392056",
  "pdf_url": null,
  "pmcid": null,
  "pmid": "42323707",
  "published": "2026-06-01",
  "publisher": "Elsevier BV",
  "ris": "https://research.draconitic.com/paper/33r8pq4fv6vax.ris",
  "sources": [
    {
      "first_seen": "2026-11-04T13:21:09Z",
      "native_id": "W7165392056",
      "source": "openalex",
      "url": "https://doi.org/10.1016/j.xplc.2026.101972"
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  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1110|Plant Molecular Biology Research",
      "subfield": "plant-biology"
    }
  ],
  "title": "Cytokinin-activated DIRIGENT13 promotes lignan synthesis to regulate root growth and abiotic stress tolerance by modulating ROS homeostasis in Arabidopsis",
  "title_text": "Cytokinin-activated DIRIGENT13 promotes lignan synthesis to regulate root growth and abiotic stress tolerance by modulating ROS homeostasis in Arabidopsis",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T13:21:09Z",
  "url": "https://research.draconitic.com/paper/33r8pq4fv6vax",
  "venue": "Plant Communications",
  "versions": []
}