{
  "abstract": "Single-stranded human telomeric DNA can fold into G-quadruplexes, structures composed of stacked G-quartets. Ligands that target G-quadruplex DNA have received considerable attention because of their potential anticancer activity. We developed a Förster resonance energy transfer (FRET) system to identify drugs that stabilize G-quadruplexes with human telomeric DNA in a homogeneous medium using Cu-4,4,4,4-(Porphine-5,10,15,20-tetrayl) tetrakis (benzoic acid)/reduced graphene oxide (Cu-TCPP/rGO) as fluorescence quencher. Fluorescein-tagged human telomeric G-rich DNA (F-GDNA) exhibited significant fluorescence quenching upon adsorption onto the Cu-TCPP/rGO surface, with fluorescence recovery observed when the ligands were bound to the G-quadruplexes. The ligand stabilized formation of G-quadruplexes was further confirmed using circular dichroism (CD) spectroscopy. Notably, flavonoids and anthraquinones derived from traditional Chinese medicines promoted G-quadruplex formation within the GDNA. The combined results of fluorescence and CD spectroscopy indicate that the proposed FRET-based methodology serves as a cost-effective, facile, and efficient tool for screening G-quadruplex ligands. This approach has significant potential for application in the identification of telomerase-targeting anticancer drugs.",
  "abstract_shown": true,
  "affiliations": [
    "Xi’an International University , , , ,",
    "Northwest University , , , ,"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 3,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Guozhen Chen",
      "orcid": "0009-0001-9387-8220"
    },
    {
      "affs": [
        0
      ],
      "name": "Jiaxin Li"
    },
    {
      "affs": [
        1
      ],
      "name": "Jianbin Zheng",
      "orcid": "0000-0002-2114-6434"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/33se90qmejnzt.bib",
  "cited_by": 0,
  "doi": "10.1021/acsomega.6c02294",
  "fulltext_url": "https://europepmc.org/articles/PMC13688510",
  "id": "33se90qmejnzt",
  "issns": [
    "2470-1343"
  ],
  "landing_url": "https://pubs.acs.org/acsodf/article/11/41/62533/5448473/A-Fluorescent-Aptamer-Sensor-Based-on-Cu-TCPP-rGO",
  "language": "eng",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|DNA and Nucleic Acid Chemistry"
    ]
  ],
  "openalex": "W7221334933",
  "pdf_url": "https://pubs.acs.org/doi/pdf/10.1021/acsomega.6c02294",
  "pmcid": "PMC13688510",
  "pmid": "42923353",
  "published": "2026-10-10",
  "publisher": "American Chemical Society (ACS)",
  "ris": "https://research.draconitic.com/paper/33se90qmejnzt.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:39Z",
      "native_id": "10.1021/acsomega.6c02294",
      "source": "crossref",
      "url": "https://pubs.acs.org/acsodf/article/11/41/62533/5448473/A-Fluorescent-Aptamer-Sensor-Based-on-Cu-TCPP-rGO"
    },
    {
      "first_seen": "2026-10-30T12:20:33Z",
      "native_id": "MED:42923353",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42923353"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|DNA and Nucleic Acid Chemistry",
      "subfield": "molecular-biology"
    }
  ],
  "title": "A Fluorescent Aptamer Sensor Based on Cu-TCPP/rGO for Homogeneous Screening of G-Quadruplex Ligands",
  "title_text": "A Fluorescent Aptamer Sensor Based on Cu-TCPP/rGO for Homogeneous Screening of G-Quadruplex Ligands",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:20:33Z",
  "url": "https://research.draconitic.com/paper/33se90qmejnzt",
  "venue": "ACS Omega",
  "versions": []
}