{
  "abstract": "Age-related keratinocyte dysfunction has been associated with altered glycosylation and dysregulated expression of several glycogenes, including St6gal1, St3gal2, and Man1a. Modulation of these aging-associated glycogenes may therefore represent a potential approach for investigating cellular aging phenotypes. In this study, an in vitro aging-like keratinocyte model exhibiting increased expression of aging-associated glycogenes was established. Antisense oligonucleotides (ASOs) targeting the upregulated glycogenes were designed and evaluated using different oligonucleotide chemistries and delivery systems. Target gene expression, lectin-based glycan profiles, Lgr6 expression, and wound closure were assessed following ASO treatment. Among the ASOs tested, phosphorodiamidate morpholino (PMO)-linked Man1a ASOs demonstrated greater suppression of Man1a mRNA than ASOs targeting St6gal1 or St3gal2. Delivery of PMO-Man1a ASOs using milk-derived nanovesicles (NV) was associated with the greatest reduction in Man1a mRNA levels compared with commercial transfection reagents. Although no significant changes were detected in ConA-reactive mannose-rich glycans, treatment was associated with an increased proportion of Lgr6⁺ cells and accelerated wound closure in aging-like keratinocytes. Overall, this study provides proof-of-concept evidence that NV-mediated delivery of glycogene-targeting ASOs can modulate selected aging-associated phenotypes in keratinocytes.",
  "abstract_shown": true,
  "affiliations": [
    "Laboratory of BioMolecular Imaging, Molecular and Cellular Biology, Department of Radiologic Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 2,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Bhanubhong Prommalee"
    },
    {
      "affs": [
        0
      ],
      "name": "Chalermchai Pilapong"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/35dvgsaqgcfh0.bib",
  "cited_by": 0,
  "doi": "10.7150/ntno.135510",
  "fulltext_url": "https://europepmc.org/articles/PMC13686789",
  "id": "35dvgsaqgcfh0",
  "issns": [
    "2206-7418"
  ],
  "landing_url": "https://europepmc.org/article/MED/42910138",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2737|Telomeres, Telomerase, and Senescence"
    ]
  ],
  "openalex": "W7222897543",
  "pdf_url": "https://europepmc.org/articles/PMC13686789?pdf=render",
  "pmcid": "PMC13686789",
  "pmid": "42910138",
  "published": "2026-10-09",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/35dvgsaqgcfh0.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:20:10Z",
      "native_id": "MED:42910138",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42910138"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2737|Telomeres, Telomerase, and Senescence",
      "subfield": "physiology"
    }
  ],
  "title": "Modulating aging-associated phenotypes in keratinocytes using antisense oligonucleotides targeting glycogenes.",
  "title_text": "Modulating aging-associated phenotypes in keratinocytes using antisense oligonucleotides targeting glycogenes.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:20:10Z",
  "url": "https://research.draconitic.com/paper/35dvgsaqgcfh0",
  "venue": "Nanotheranostics",
  "versions": []
}