{
  "abstract": "Itaconic acid (IA) is an important bio-based platform chemical with applications in the production of polymers, coatings, resins, and other value-added materials. However, its conventional microbial production relies predominantly on microorganisms that present safety and process-related limitations. This study investigated Saccharomyces cerevisiae BY4742 as a potentially safer microbial platform for the production of bio-based IA under submerged fermentation conditions. IA production was optimized using response surface methodology with a Box–Behnken design, considering glucose concentration, initial pH, and CaCI2 concentration as independent variables. The maximum IA concentration of 1133.50 ± 54.92 mg/L was obtained at 30 °C, pH 4.5, with 10% (w/v) glucose and without CaCI2 supplementation after 216 h of fermentation. The fermentation-derived IA was subsequently recovered and characterized using high-performance liquid chromatography, Fourier-transform infrared spectroscopy, 1H-nuclear magnetic resonance (1H-NMR), and thermogravimetric analysis. The analytical results confirmed the molecular identity and high chromatographic purity of the recovered IA. Furthermore, the homopolymerization ability of the fermentation-derived IA was evaluated and compared with that of commercial IA under the same reaction conditions. The 1H-NMR results demonstrated changes consistent with consumption of the IA C=C double bond following polymerization, indicating that the fermentation-derived IA retained its ability to undergo homopolymerization. Overall, these findings demonstrate the potential of S. cerevisiae for producing structurally confirmed bio-based IA and provide a proof of concept for its use as a renewable monomer for poly(itaconic acid) synthesis, supporting its further investigation for the development of bio-based polymeric materials.",
  "abstract_shown": true,
  "affiliations": [
    "Science and Technology Application and Research Center, Dokuz Eylul University, 35390 Buca, Türkiye",
    "Biochemistry Division, Department of Chemistry, Faculty of Science, Dokuz Eylul University, 35390 Buca, Türkiye",
    "Biotechnology Department, The Graduate School of Natural and Applied Sciences, Dokuz Eylul University, 35390 Buca, Türkiye",
    "Leather Engineering Department, Faculty of Engineering, Ege University, 35100 Bornova, Türkiye"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Zulfiye Velioglu Tosuner",
      "orcid": "0000-0001-9181-6619"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Mehdi Kosaca",
      "orcid": "0000-0003-2075-2480"
    },
    {
      "affs": [
        1
      ],
      "name": "Raziye Ozturk Urek",
      "orcid": "0000-0002-7147-6853"
    },
    {
      "affs": [
        3
      ],
      "name": "Onur Yilmaz",
      "orcid": "0000-0002-0992-5937"
    },
    {
      "affs": [
        1
      ],
      "name": "Catalina Natalia Yilmaz",
      "orcid": "0000-0001-5229-8986"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/35ezw1pbfkpsy.bib",
  "cited_by": 0,
  "doi": "10.3390/ijms27198923",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13666190/?tool=EBI",
  "id": "35ezw1pbfkpsy",
  "issns": [
    "1422-0067"
  ],
  "landing_url": "https://europepmc.org/article/PMC/PMC13666190",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Microbial Metabolic Engineering and Bioproduction"
    ]
  ],
  "openalex": "W7221180780",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13666190/pdf/?tool=EBI",
  "pmcid": "PMC13666190",
  "pmid": null,
  "published": "2026-10-01",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/35ezw1pbfkpsy.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:31Z",
      "native_id": "PMC:PMC13666190",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13666190"
    },
    {
      "first_seen": "2026-10-30T12:18:55Z",
      "native_id": "10.3390/ijms27198923",
      "source": "crossref",
      "url": "https://www.mdpi.com/1422-0067/27/19/8923"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Microbial Metabolic Engineering and Bioproduction",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Production, Optimization and Characterization of Biobased Itaconic Acid from Saccharomyces cerevisiae",
  "title_text": "Production, Optimization and Characterization of Biobased Itaconic Acid from Saccharomyces cerevisiae",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:55Z",
  "url": "https://research.draconitic.com/paper/35ezw1pbfkpsy",
  "venue": "International journal of molecular sciences",
  "versions": []
}