{
  "abstract": "Cells throughout the body release small extracellular vesicles (sEVs), including exosomes, that function as modulators of both local and systemic metabolism. Using mice in which ALIX, a key regulator of sEV biogenesis, was selectively deleted in PDGFRα + mesenchymal progenitors (Pα-ALIXKO mice), we demonstrate a critical role for mesenchymal sEV production in metabolic regulation. In high-fat diet–induced obesity, Pα-ALIXKO mice exhibited reduced weight gain and smaller subcutaneous adipocytes compared with control mice. Despite similar insulin sensitivity, these mice showed impaired glucose tolerance, which was associated with reduced pancreatic β cell area and smaller islets. sEVs isolated from conditioned media of knockout (KO)–derived mesenchymal stem cells (MSCs) exhibited a reduced capacity to promote β cell proliferation. Furthermore, MSCs isolated from subcutaneous adipose tissue of KO mice showed elevated mRNA expression of inflammatory genes. Together, these findings indicate that ALIX in tissue-resident mesenchymal progenitors is required for pancreatic β cell proliferation and glucose homeostasis in obesity.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Metabolic Medicine, Graduate School of Medicine, The University of Osaka, 2-2, Yamadaoka, Suita, Osaka 565-0871, Japan.",
    "Department of Metabolism and Atherosclerosis, Graduate School of Medicine, The University of Osaka, 2-2, Yamadaoka, Suita, Osaka 565-0871, Japan."
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 12,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Yuhei Uehara",
      "orcid": "0009-0008-8739-0721"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Hirofumi Nagao",
      "orcid": "0009-0001-1261-4160"
    },
    {
      "affs": [
        0
      ],
      "name": "Aoki Tobimatsu"
    },
    {
      "affs": [
        0
      ],
      "name": "Keitaro Kawada",
      "orcid": "0000-0003-4976-2121"
    },
    {
      "affs": [
        0
      ],
      "name": "Shunsuke Shiode",
      "orcid": "0009-0007-7682-0602"
    },
    {
      "affs": [
        0
      ],
      "name": "Emi Horitani",
      "orcid": "0000-0003-1434-9214"
    },
    {
      "affs": [
        0
      ],
      "name": "Yoshinari Obata",
      "orcid": "0000-0002-3443-3635"
    },
    {
      "affs": [
        0
      ],
      "name": "Shiro Fukuda",
      "orcid": "0000-0003-0712-7154"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Yuya Fujishima",
      "orcid": "0000-0003-0028-1757"
    },
    {
      "affs": [
        0
      ],
      "name": "Shunbun Kita",
      "orcid": "0000-0002-8937-0053"
    },
    {
      "affs": [
        0
      ],
      "name": "Hitoshi Nishizawa"
    },
    {
      "affs": [
        0
      ],
      "name": "Iichiro Shimomura"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/35gsyxmn1n7t0.bib",
  "cited_by": 0,
  "doi": "10.1126/sciadv.aei1333",
  "fulltext_url": null,
  "id": "35gsyxmn1n7t0",
  "issns": [
    "2375-2548"
  ],
  "landing_url": "https://www.science.org/doi/10.1126/sciadv.aei1333",
  "language": null,
  "licence": "CC-BY-NC",
  "licence_label": "CC BY-NC",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Extracellular vesicles in disease"
    ]
  ],
  "openalex": "W7221073953",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-09",
  "publisher": "American Association for the Advancement of Science (AAAS)",
  "ris": "https://research.draconitic.com/paper/35gsyxmn1n7t0.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:41Z",
      "native_id": "10.1126/sciadv.aei1333",
      "source": "crossref",
      "url": "https://www.science.org/doi/10.1126/sciadv.aei1333"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Extracellular vesicles in disease",
      "subfield": "molecular-biology"
    }
  ],
  "title": "ALIX-dependent small extracellular vesicles from tissue-resident mesenchymal cells regulate pancreatic β cell proliferation in obesity",
  "title_text": "ALIX-dependent small extracellular vesicles from tissue-resident mesenchymal cells regulate pancreatic β cell proliferation in obesity",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:41Z",
  "url": "https://research.draconitic.com/paper/35gsyxmn1n7t0",
  "venue": "Science Advances",
  "versions": []
}