{
  "abstract": "Liquid biopsy using circulating tumor DNA (ctDNA) is increasingly used for cancer profiling and monitoring, but blood-based analysis may not capture tumor heterogeneity across anatomical compartments. We evaluated cell-free DNA from cerebrospinal (n = 7), pleural (n = 11), pericardial (n = 1), and ascitic (n = 26) fluids from 45 patients with advanced cancer, comparing molecular findings with matched tissue, blood, and cytology where available. Oncogenic alterations were detected in 87% of body fluid samples, with a median variant allele frequency of 3.15%. TP53 (49%), EGFR (18%), and KRAS (18%) were the most frequently altered genes, and ESCAT tier IA alterations occurred in 31.1% of patients. In six patients (13.3%), potentially targetable alterations were detected in body fluids but not in blood and/or tissue. Among five cytology-negative samples, four were ctDNA positive. These findings support body fluid ctDNA profiling as a complementary approach to tissue, plasma, and cytology, thereby warranting prospective validation.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": "Article",
  "arxiv": null,
  "author_total": 13,
  "authors": [
    {
      "name": "Aditya Shreenivas"
    },
    {
      "name": "Sewanti Limaye"
    },
    {
      "name": "Massimo Cristofanilli"
    },
    {
      "name": "Matt Lechner"
    },
    {
      "name": "Andrew M. Gaya"
    },
    {
      "name": "Tim Crook"
    },
    {
      "name": "Pollyanna Leite"
    },
    {
      "name": "Kefah Mokbel"
    },
    {
      "name": "Dorthe Schaffrin-Nabe"
    },
    {
      "name": "Humaid O. Al-Shamsi"
    },
    {
      "name": "Darshana Patil",
      "orcid": "0009-0002-5593-6132"
    },
    {
      "name": "Rajan Datar"
    },
    {
      "name": "Razelle Kurzrock"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/35jvmkrw9hb7x.bib",
  "cited_by": 0,
  "doi": "10.1016/j.isci.2026.117893",
  "fulltext_url": "https://europepmc.org/articles/PMC13694802",
  "id": "35jvmkrw9hb7x",
  "issns": [
    "2589-0042"
  ],
  "landing_url": "https://www.cell.com/iscience/fulltext/S2589-0042(26)03276-1",
  "language": "eng",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "http://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Genomics"
    ],
    [
      "heading",
      "Molecular biology"
    ],
    [
      "openalex",
      "1306|Cancer Genomics and Diagnostics"
    ]
  ],
  "openalex": "W7221829521",
  "pdf_url": "https://europepmc.org/articles/PMC13694802?pdf=render",
  "pmcid": "PMC13694802",
  "pmid": "42926854",
  "published": "2026-10-14",
  "publisher": "Elsevier BV",
  "ris": "https://research.draconitic.com/paper/35jvmkrw9hb7x.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:16Z",
      "native_id": "10.1016/j.isci.2026.117893",
      "source": "feed-cell",
      "url": "https://www.cell.com/iscience/fulltext/S2589-0042(26)03276-1"
    },
    {
      "first_seen": "2026-10-30T12:17:49Z",
      "native_id": "10.1016/j.isci.2026.117893",
      "source": "crossref",
      "url": "https://linkinghub.elsevier.com/retrieve/pii/S2589004226032761"
    },
    {
      "first_seen": "2026-10-30T12:21:18Z",
      "native_id": "MED:42926854",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42926854"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "heading:Molecular biology",
      "subfield": "molecular-biology"
    },
    {
      "field": "biology",
      "origin": "heading:Genomics",
      "subfield": "genetics-genomics"
    }
  ],
  "title": "Circulating tumor DNA in body fluids for molecular characterization of cancers beyond tissue and blood",
  "title_text": "Circulating tumor DNA in body fluids for molecular characterization of cancers beyond tissue and blood",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:21:18Z",
  "url": "https://research.draconitic.com/paper/35jvmkrw9hb7x",
  "venue": "iScience",
  "versions": []
}