{
  "abstract": "The receptor for advanced glycation end products (RAGE/AGER) is an important mediator of inflammatory signaling and has been implicated in chronic low-grade inflammation associated with aging. Chronic low-grade inflammation (“inflammaging”) is a characteristic feature of biological aging, providing a rationale for investigating molecular regulators of inflammatory signaling such as RAGE. This study aimed to develop and validate short hairpin RNA (shRNA) constructs suppressing AGER/RAGE in human cell models. Five shRNA constructs targeting human AGER were designed, cloned into pLSL-puro, and verified by Sanger sequencing. Lentiviral vectors were produced in HEK293T cells and used to establish stable HEK293T, 786-O, and human umbilical vein endothelial cell (HUVEC) populations with AGER knockdown. AGER/RAGE expression was assessed by RT-qPCR, Western blotting, and flow cytometry. Silencing efficiency varied with the construct and the cellular model. sh1 produced the strongest suppression of AGER, exceeding 70% in selected models, whereas sh2 was effective in HEK293T and 786-O cells. Reduced RAGE expression was confirmed at the mRNA, protein, and cell-surface levels, identifying sh1 and sh2 as lead candidates. An AGER-focused workflow for lentiviral shRNA construct screening and experimental validation was established, providing a reproducible approach for generating stable cell models with reduced AGER/RAGE expression. Further work is required to confirm isoform specificity, assess off-target effects, and define the functional consequences of RAGE suppression.",
  "abstract_shown": true,
  "affiliations": [
    "Institute of Biology of Aging and Medicine of Healthy Longevity with the Clinic of Predictive Medicine, Petrovsky National Research Centre of Surgery, 119435 Moscow, Russia"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 3,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Tatiana A. Muzaffarova"
    },
    {
      "affs": [
        0
      ],
      "name": "Elena M. Malinovskaya"
    },
    {
      "affs": [
        0
      ],
      "name": "Alexey Moskalev"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/37ac0dz132j3e.bib",
  "cited_by": 0,
  "doi": "10.3390/ijms27209167",
  "fulltext_url": null,
  "id": "37ac0dz132j3e",
  "issns": [
    "1422-0067"
  ],
  "landing_url": "https://www.mdpi.com/1422-0067/27/20/9167",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1308|Advanced Glycation End Products research"
    ]
  ],
  "openalex": "W7222628286",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-16",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/37ac0dz132j3e.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:57Z",
      "native_id": "10.3390/ijms27209167",
      "source": "crossref",
      "url": "https://www.mdpi.com/1422-0067/27/20/9167"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:1308|Advanced Glycation End Products research",
      "subfield": "general"
    }
  ],
  "title": "RNA Interference-Mediated Suppression of RAGE",
  "title_text": "RNA Interference-Mediated Suppression of RAGE",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:57Z",
  "url": "https://research.draconitic.com/paper/37ac0dz132j3e",
  "venue": "International Journal of Molecular Sciences",
  "versions": []
}