{
  "abstract": "To characterize age-associated molecular differences in the longissimus dorsi muscle of female Ashdan yaks, this study combined Oxford Nanopore Technologies (ONT) full-length transcriptomic sequencing and untargeted LC-MS metabolomics in 27 independent animals aged 10 days (M0), 6 months (M6), and 54 months (M54), with nine animals per group. At BH q < 0.05 and |log2FC| > 1, the three pairwise comparisons identified 1999, 3480, and 2350 differentially expressed genes, respectively, with the largest set between M0 and M54. Networks reconstructed from these gene sets identified high-connectivity candidates, including ENSBGRG00000003669 in comparisons involving M0 and MRTO4 and BYSL in M6 vs. M54. Of 1842 retained annotated LC-MS peak features, 1487, 1474, and 1285 were differential at BH q < 0.05; 676, 589, and 452 also showed at least twofold differences. Separate gene-overrepresentation and quantitative metabolite member-profile tests supported oxidative phosphorylation in both comparisons involving M0 and purine metabolism in M0 vs. M6. Alanine, aspartate and glutamate metabolism showed recurring metabolite-profile differences without significant gene overrepresentation. Among 1860 matched gene-feature pairs, 977 pooled associations passed BH q < 0.05. The transcript resource included 4642 novel candidates and 14 unique differential splicing candidates. These findings characterize age-associated transcriptional and metabolic variation and prioritize pathways and computational candidates for subsequent study.",
  "abstract_shown": true,
  "affiliations": [
    "Key Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China",
    "Key Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China",
    "Institute of Western Agriculture, Chinese Academy of Agricultural Sciences, Changji 831100, China"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Jingying Deng"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Qingbo Zheng"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Xiaoyun Wu"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Chao Chen"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Pengjia Bao",
      "orcid": "0000-0001-7671-1050"
    },
    {
      "affs": [
        2
      ],
      "name": "Ning Li"
    },
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Ping Yan"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/37axsnps5z3zm.bib",
  "cited_by": 0,
  "doi": "10.3390/biom16111591",
  "fulltext_url": null,
  "id": "37axsnps5z3zm",
  "issns": [
    "2218-273X"
  ],
  "landing_url": "https://www.mdpi.com/2218-273X/16/11/1591",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [],
  "openalex": null,
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-11-02",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/37axsnps5z3zm.ris",
  "sources": [
    {
      "first_seen": "2026-11-03T00:23:55Z",
      "native_id": "10.3390/biom16111591",
      "source": "crossref",
      "url": "https://www.mdpi.com/2218-273X/16/11/1591"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "issn:2218-273X",
      "subfield": "general"
    }
  ],
  "title": "Comparative Full-Length Transcriptomic and Untargeted Metabolomic Profiling of Longissimus Dorsi Muscle in Female Ashdan Yaks at Three Postnatal Ages",
  "title_text": "Comparative Full-Length Transcriptomic and Untargeted Metabolomic Profiling of Longissimus Dorsi Muscle in Female Ashdan Yaks at Three Postnatal Ages",
  "type": "peer-reviewed",
  "updated_at": "2026-11-04T00:24:50Z",
  "url": "https://research.draconitic.com/paper/37axsnps5z3zm",
  "venue": "Biomolecules",
  "versions": []
}