{
  "abstract": "Purpose: To investigate genetic material in biopsy-related droplets (BRDs) from blastocyst biopsy and its potential applications in preimplantation genetic testing for structural rearrangements (PGT-SR).\n\nMethods: BRDs were collected from 218 blastocysts by combining three types of biopsy-related droplets (biopsy, washing, and temporary storage droplets) into ~15 µL samples. Genomic DNA amplification and copy number variation (CNV) sequencing library preparation were performed. Next-generation sequencing (NGS) was conducted on BRD libraries with concentrations ≥0.5 ng/µL.\n\nResults: 69.27% of BRDs had a sequencing library concentration (CSL) ≥ 0.5 ng/µL, with a CNV detection success rate of 94.04%. CSL was significantly higher in biopsy-classified non-euploid than euploid blastocysts (P < 0.0001). Among 142 BRDs with successful CNV detection, 87.32% showed ploidy concordance with PGT findings. Complete or partial concordance was observed in 77.53% of blastocysts with structural rearrangement-related abnormalities. In 37 transferred biopsy-euploid blastocysts, clinical pregnancy rates were higher in the BRD-euploid and undetectable/non-informative BRD groups than in the BRD-non-euploid group (85.71% and 86.96% vs. 28.57%). In seven blastocysts undergoing secondary biopsy after initial testing failure, BRD and secondary biopsy results showed 85.71% ploidy concordance.\n\nConclusions: BRD-based genetic testing may serve as an adjunct to conventional biopsy-based testing in PGT-SR.",
  "abstract_shown": true,
  "affiliations": [
    "Center of Reproductive Medicine, Fujian Maternity and Child Health Hospital",
    "Fujian Maternity and Child Health Hospital College of Clinical Medicine for Obstetrics and Gynecology and Pediatrics, Fujian Medical University",
    "The School of Basic Medical Sciences, Fujian Medical University",
    "The First Clinical Medical College, Fujian Medical University",
    "Fujian Clinical Research Center for Maternal-Fetal Medicine"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Zhiqing Huang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Yuzhi Chen"
    },
    {
      "affs": [
        2
      ],
      "name": "Xiaonuan Cai"
    },
    {
      "affs": [
        3
      ],
      "name": "Shujing Zhu"
    },
    {
      "affs": [
        3
      ],
      "name": "Jing Wang"
    },
    {
      "affs": [
        2
      ],
      "name": "Ruili Xie"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Hang Shi"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Aili Yu"
    },
    {
      "affs": [
        0,
        4
      ],
      "name": "Beihong Zheng"
    },
    {
      "affs": [
        0,
        4
      ],
      "name": "Yan Sun"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/38g9we77b65qk.bib",
  "cited_by": 0,
  "doi": "10.3389/fendo.2026.1946046",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13730871/",
  "id": "38g9we77b65qk",
  "issns": [
    "1664-2392"
  ],
  "landing_url": "https://www.frontiersin.org/articles/10.3389/fendo.2026.1946046/full",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Original Research"
    ],
    [
      "openalex",
      "2735|Prenatal Screening and Diagnostics"
    ]
  ],
  "openalex": "W7223124524",
  "pdf_url": "https://www.frontiersin.org/journals/endocrinology/articles/10.3389/fendo.2026.1946046/pdf",
  "pmcid": "PMC13730871",
  "pmid": "42984540",
  "published": "2026-10-21",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/38g9we77b65qk.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:20:20Z",
      "native_id": "10.3389/fendo.2026.1946046",
      "source": "crossref",
      "url": "https://www.frontiersin.org/articles/10.3389/fendo.2026.1946046/full"
    },
    {
      "first_seen": "2026-11-07T12:23:50Z",
      "native_id": "PMC13730871",
      "source": "pmc",
      "url": "https://doi.org/10.3389/fendo.2026.1946046"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2735|Prenatal Screening and Diagnostics",
      "subfield": "reproductive-child"
    }
  ],
  "title": "Genetic analysis of biopsy-related droplets and its potential applications in PGT-SR patients",
  "title_text": "Genetic analysis of biopsy-related droplets and its potential applications in PGT-SR patients",
  "type": "peer-reviewed",
  "updated_at": "2026-11-08T12:21:11Z",
  "url": "https://research.draconitic.com/paper/38g9we77b65qk",
  "venue": "Frontiers in Endocrinology",
  "versions": []
}