{
  "abstract": "Purpose\n\nThe purpose of this study was to investigate the dynamic immune response and the role of DECTIN-1 in Acanthamoeba keratitis (AK).Methods\n\nThe AK mouse model was established, and corneal tissue samples were collected at 1, 3, and 7 days post-infection (dpi). Transcriptomic profiling was performed to analyze differentially expressed genes and enriched Gene Ontology terms. Immune cell infiltration was estimated using ImmuCellAI-mouse. The expression of DECTIN-1 and associated signaling molecules was validated using quantitative PCR, immunostaining, and Western blotting. In vitro experiments were conducted to assess DECTIN-1 expression in human corneal epithelial cells (HCECs).Results\n\nAK progression was characterized by dynamic changes, with peak inflammatory responses at 3 dpi. Immune infiltration analysis revealed early innate immune activation followed by adaptive immune involvement at later stages. Temporal transcriptome analysis identified DECTIN-1 as a significantly upregulated gene throughout disease progression. Activation of the DECTIN-1/SYK/NF-κB signaling pathway was associated with increased expression of pro-inflammatory cytokines. In vitro experiments confirmed that Acanthamoeba directly induced DECTIN-1 expression in HCECs.Conclusions\n\nDECTIN-1 was dynamically upregulated during AK and may play a potential role in mediating innate immune responses and shaping immune cell dynamics. These findings provided new insights into AK pathogenesis and indicated DECTIN-1 as a potential therapeutic target.",
  "abstract_shown": true,
  "affiliations": [
    "Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing, People's Republic of China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 12,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Jinding Pang"
    },
    {
      "affs": [
        0
      ],
      "name": "Zhenyu Wei"
    },
    {
      "affs": [
        0
      ],
      "name": "Yuheng Zhang"
    },
    {
      "affs": [
        0
      ],
      "name": "Yuan Wei"
    },
    {
      "affs": [
        0
      ],
      "name": "Qingquan Shi"
    },
    {
      "affs": [
        0
      ],
      "name": "Qiankun Chen"
    },
    {
      "affs": [
        0
      ],
      "name": "Zijun Zhang"
    },
    {
      "affs": [
        0
      ],
      "name": "Sitong Shen"
    },
    {
      "affs": [
        0
      ],
      "name": "Bo Peng"
    },
    {
      "affs": [
        0
      ],
      "name": "Ahyan Ilman Qudsi"
    },
    {
      "affs": [
        0
      ],
      "name": "Sizhe Ji Wu"
    },
    {
      "affs": [
        0
      ],
      "name": "Qingfeng Liang"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/38t3x7wv9mz3j.bib",
  "cited_by": 0,
  "doi": "10.1167/iovs.67.12.25",
  "fulltext_url": "https://europepmc.org/articles/PMC13676222",
  "id": "38t3x7wv9mz3j",
  "issns": [
    "0146-0404",
    "1552-5783"
  ],
  "landing_url": "https://europepmc.org/article/MED/42872947",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2731|Ocular Infections and Treatments"
    ]
  ],
  "openalex": "W7221818477",
  "pdf_url": "https://europepmc.org/articles/PMC13676222?pdf=render",
  "pmcid": "PMC13676222",
  "pmid": "42872947",
  "published": "2026-10-01",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/38t3x7wv9mz3j.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:00Z",
      "native_id": "MED:42872947",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42872947"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2731|Ocular Infections and Treatments",
      "subfield": "general"
    }
  ],
  "title": "Temporal Transcriptomic Profiling Reveals DECTIN-1-Mediated Immune Responses in Acanthamoeba Keratitis.",
  "title_text": "Temporal Transcriptomic Profiling Reveals DECTIN-1-Mediated Immune Responses in Acanthamoeba Keratitis.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:00Z",
  "url": "https://research.draconitic.com/paper/38t3x7wv9mz3j",
  "venue": "Investigative ophthalmology & visual science",
  "versions": []
}