{
  "abstract": "The global demand for renewable liquid fuels and biobased chemicals has intensified interest in engineering high‐biomass C4 grasses to accumulate triacylglycerol (TAG) in vegetative tissues. Achieving this goal requires coordinated solutions across molecular biology, crop science, chemical engineering, and life cycle assessment, disciplines that rarely operate within a single research program. The Center for Advanced Bioenergy and Bioproducts Innovation (CABBI) was established to provide this integration through a Design–Build–Test–Learn (DBTL) framework applied to three C4 feedstocks: saccharum (Saccharum spp. hybrids), sorghum ( Sorghum bicolor ), and miscanthus (Miscanthus × giganteus). Through iterative DBTL cycles, CABBI has produced transgenic saccharum and sorghum lines with substantially elevated levels of vegetative TAG, with multi‐location field trials having confirmed stable trait heritability and lipid yields. We have also leveraged our integration to develop a dual system biorefinery that co‐recovers vegetative lipids with fermentable sugars at levels sufficient to achieve industrial ethanol titres. Importantly, this fully integrated cross‐disciplinary data integration model has highlighted the key constraints currently preventing TAG‐rich vegetative oil from achieving commercial viability as: (i) active lipid turnover, (ii) photosynthetic trade‐offs, (iii) spatial misexpression, and (iv) bioprocessing challenges. CABBI uses emerging technologies, including biofoundry‐enabled construct screening, precision genome editing, and machine‐learning‐assisted regulatory design, to further accelerate DBTL cycles. More broadly, this work illustrates how integrative centre‐scale research that unites diverse expertise can accelerate discovery and translation, positioning these systems to expand agricultural markets and contribute to a more secure and resilient bioeconomy.",
  "abstract_shown": true,
  "affiliations": [
    "Carl R. Woese Institute of Genomic Biology University of Illinois Urbana‐Champaign Urbana Illinois USA",
    "Department of Plant Biology University of Illinois Urbana‐Champaign Urbana Illinois USA",
    "Center for Digital Agriculture University of Illinois Urbana‐Champaign Urbana Illinois USA",
    "DOE Center for Advanced Bioenergy and Bioproducts Innovation University of Illinois Urbana‐Champaign Urbana Illinois USA",
    "Center for Plant Science Innovation and Department of Biochemistry University of Nebraska‐Lincoln Lincoln Nebraska USA",
    "DOE Center for Advanced Bioenergy and Bioproducts Innovation University of Nebraska‐Lincoln Lincoln Nebraska USA",
    "Department of Agricultural and Biological Engineering University of Illinois Urbana‐Champaign Urbana Illinois USA",
    "Plant Breeding and Genetics Section, School of Integrative Plant Science Cornell University Ithaca New York USA",
    "HudsonAlpha Institute for Biotechnology Huntsville Alabama USA",
    "DOE Center for Advanced Bioenergy and Bioproducts Innovation HudsonAlpha Institute for Biotechnology Huntsville Alabama USA",
    "The Grainger College of Engineering, Department of Civil and Environmental Engineering University of Illinois Urbana‐Champaign Urbana Illinois USA",
    "Department of Agronomy & Horticulture University of Nebraska‐Lincoln Lincoln Nebraska USA",
    "Plant Transformation Core Research Facility, Agricultural Research Division, Institute of Agriculture and Natural Resources University of Nebraska‐Lincoln Lincoln Nebraska USA",
    "Department of Biology Brookhaven National Laboratory Upton New York USA",
    "DOE Center for Advanced Bioenergy and Bioproducts Innovation Brookhaven National Laboratory Upton New York USA",
    "Agronomy Department, Plant Molecular and Cellular Biology Program, Genetics Institute University of Florida, Institute of Food and Agricultural Sciences Gainesville Florida USA",
    "DOE Center for Advanced Bioenergy and Bioproducts Innovation University of Florida Gainesville Florida USA"
  ],
  "article_type": "review article",
  "arxiv": null,
  "author_total": 13,
  "authors": [
    {
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        0,
        1,
        2,
        3
      ],
      "name": "Daniel Lunn",
      "orcid": "0000-0002-9386-1568"
    },
    {
      "affs": [
        4,
        5
      ],
      "name": "Kiyoul Park",
      "orcid": "0000-0002-0711-0398"
    },
    {
      "affs": [
        3,
        6
      ],
      "name": "Narendra Naik Deshavath",
      "orcid": "0000-0002-7432-7570"
    },
    {
      "affs": [
        0,
        1,
        3,
        7
      ],
      "name": "Jia Dong",
      "orcid": "0000-0002-9961-8446"
    },
    {
      "affs": [
        8,
        9
      ],
      "name": "Kankshita Swaminathan",
      "orcid": "0000-0002-4936-509X"
    },
    {
      "affs": [
        3,
        10
      ],
      "name": "Jeremy Guest",
      "orcid": "0000-0003-2489-2579"
    },
    {
      "affs": [
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        1,
        2,
        3
      ],
      "name": "Andrew D. B. Leakey",
      "orcid": "0000-0001-6251-024X"
    },
    {
      "affs": [
        5,
        11,
        12
      ],
      "name": "Nathaniel M. Butler",
      "orcid": "0000-0002-9235-7626"
    },
    {
      "affs": [
        13,
        14
      ],
      "name": "Jorg Schwender",
      "orcid": "0000-0003-1350-4171"
    },
    {
      "affs": [
        3,
        6
      ],
      "name": "Vijay Singh",
      "orcid": "0000-0003-4349-8681"
    },
    {
      "affs": [
        13,
        14
      ],
      "name": "John Shanklin",
      "orcid": "0000-0002-6774-8043"
    },
    {
      "affs": [
        15,
        16
      ],
      "name": "Fredy Altpeter",
      "orcid": "0000-0002-0894-4976"
    },
    {
      "affs": [
        4,
        5
      ],
      "name": "Edgar B. Cahoon",
      "orcid": "0000-0002-7277-1176"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/3a09s747v4dkn.bib",
  "cited_by": 0,
  "doi": "10.1111/pbi.70771",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13714591/",
  "id": "3a09s747v4dkn",
  "issns": [
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    "1467-7652"
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  "landing_url": "https://doi.org/10.1111/pbi.70771",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Review Article"
    ],
    [
      "openalex",
      "1102|Bioenergy crop production and management"
    ]
  ],
  "openalex": "W7224100805",
  "pdf_url": "https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/pbi.70771",
  "pmcid": "PMC13714591",
  "pmid": "42947611",
  "published": "2026-10-28",
  "publisher": "Wiley",
  "ris": "https://research.draconitic.com/paper/3a09s747v4dkn.ris",
  "sources": [
    {
      "first_seen": "2026-11-01T12:22:03Z",
      "native_id": "PMC13714591",
      "source": "pmc",
      "url": "https://doi.org/10.1111/pbi.70771"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1102|Bioenergy crop production and management",
      "subfield": "plant-biology"
    }
  ],
  "title": "Lessons From CABBI: Advancing Vegetative Oil Production in C4 Crops Through a Vertically Integrated DBTL Framework",
  "title_text": "Lessons From CABBI: Advancing Vegetative Oil Production in C4 Crops Through a Vertically Integrated DBTL Framework",
  "type": "peer-reviewed",
  "updated_at": "2026-11-01T12:22:03Z",
  "url": "https://research.draconitic.com/paper/3a09s747v4dkn",
  "venue": "Plant Biotechnology Journal",
  "versions": []
}