{
  "abstract": "This study aimed to elucidate the impacts of cultivating different herbaceous perennials on the rhizosphere microenvironment, nitrogen-cycling microbial communities, and functional genes in black soils of urban green spaces in Northeast China, thereby providing a scientific basis for rational urban plant configuration and interspecific differences in soil nutrient cycling. Focusing on five commonly utilized herbaceous perennials in Changchun ( Lycopus lucidus [P-LL], Salvia japonica [P-SJ], Hemerocallis fulva [P-HF], Hosta ensata [P-HE], and Pseudolysimachion spicatum [P-PS]), this study integrated conventional physicochemical analyses, metagenomic sequencing, co-occurrence networks, and Random Forest modeling to analyze rhizosphere soil nutrient profiles, nitrogen-cycling microbial community structures, and key functional gene abundances after two years of continuous cultivation. Different floral treatments significantly altered rhizosphere nutrient status and microbial community composition. The P-LL treatment exhibited relatively higher contents of total nitrogen (TN), soil microbial biomass nitrogen (SMBN), available phosphorus, and available potassium, demonstrating a high-level soil multifunctionality index (SMI). The P-PS treatment showed higher available nitrogen and ammonium nitrogen but lower SMBN, accompanied by a higher relative abundance of Actinomycetota and the assembly of a microbial co-occurrence network with enhanced connectivity and robustness, indicating a potent organic nitrogen mineralization potential in its rhizosphere. The composition of nitrogen-cycling genes remained broadly stable across treatments, dominated by ammonia assimilation genes ( glnA and gltB ), with a relatively low abundance of denitrification genes ( nirK ). Multivariate and Random Forest analyses revealed that SMBN exerted the strongest positive regulatory effect on nitrogen-sensing genes (e.g., ntrY and ntrX ), whereas TN, nitrate nitrogen (NO ₃ ⁻ -N), and available potassium (AK) emerged as the paramount direct drivers determining the abundance variations of core ammonia assimilation genes. Different herbaceous perennials shaped species-specific rhizosphere nutrient and microbial functional profiles. P-LL and P-PS exhibited superior comprehensive nutrient traits and potential nitrogen mineralization characteristics, respectively, making them excellent candidate species for urban green space configuration and future mixed-planting trials in cold regions.",
  "abstract_shown": true,
  "affiliations": [
    "College of Landscape Architecture, Changchun University , ,",
    "Agronomy College, Jilin Agricultural University , ,"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Pushun Bian"
    },
    {
      "affs": [
        0
      ],
      "name": "Bo Han"
    },
    {
      "affs": [
        0
      ],
      "name": "Haifeng Zhu"
    },
    {
      "affs": [
        0
      ],
      "name": "Yu Zhou"
    },
    {
      "affs": [
        1
      ],
      "name": "Zexin Qi"
    },
    {
      "affs": [
        0
      ],
      "name": "Wei Gao"
    },
    {
      "affs": [
        0
      ],
      "name": "Yisen Wang"
    },
    {
      "affs": [
        0
      ],
      "name": "Nuo Chen"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/3a1r127r8easj.bib",
  "cited_by": 0,
  "doi": "10.3389/fpls.2026.1961532",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13672543/?tool=EBI",
  "id": "3a1r127r8easj",
  "issns": [
    "1664-462X"
  ],
  "landing_url": "https://europepmc.org/article/PMC/PMC13672543",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1111|Soil Carbon and Nitrogen Dynamics"
    ]
  ],
  "openalex": "W7216057839",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13672543/pdf/?tool=EBI",
  "pmcid": "PMC13672543",
  "pmid": null,
  "published": "2026-10-02",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/3a1r127r8easj.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:18Z",
      "native_id": "PMC:PMC13672543",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13672543"
    },
    {
      "first_seen": "2026-10-30T12:19:25Z",
      "native_id": "10.3389/fpls.2026.1961532",
      "source": "crossref",
      "url": "https://www.frontiersin.org/articles/10.3389/fpls.2026.1961532/full"
    }
  ],
  "subjects": [
    {
      "field": "earth",
      "origin": "openalex:1111|Soil Carbon and Nitrogen Dynamics",
      "subfield": "hydrology-soils"
    }
  ],
  "title": "Effects of cultivation of herbaceous perennials on soil microbial community structure and nitrogen cycling functions",
  "title_text": "Effects of cultivation of herbaceous perennials on soil microbial community structure and nitrogen cycling functions",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:25Z",
  "url": "https://research.draconitic.com/paper/3a1r127r8easj",
  "venue": "Frontiers in plant science",
  "versions": []
}