{
  "abstract": "Urinary tract infections are a major global health concern, with Klebsiella pneumoniae emerging as a prominent multidrug-resistant uropathogen. The increasing prevalence of extended-spectrum β-lactamase-producing strains has reduced the effectiveness of β-lactam antibiotics. In this study, 87 clinical K. pneumoniae isolates from India were analysed using whole-genome sequencing, MLST and antimicrobial resistance profiling. β-Lactam resistance was the dominant phenotype, with blaSHV and blaCTX-M identified as the most prevalent class A β-lactamase genes. Molecular docking and 100 ns molecular dynamics simulations were conducted to examine enzyme-antibiotic interactions. Meropenem exhibited the lowest binding affinity with both proteins (-2.695 kcal mol⁻¹ for blaSHV and -1.849 kcal mol⁻¹ for blaCTX-M), forming unstable complexes, particularly with blaCTX-M. These findings highlight the growing challenge of β-lactam resistance and emphasize the need for genomic surveillance and alternative therapeutic strategies against multidrug-resistant K. pneumoniae.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Bioinformatics, Pondicherry University, Puducherry, India.",
    "Ciencias Agrogenómicas, Escuela Nacional de Estudios Superiores Unidad León, Universidad Nacional Autónoma de México (UNAM), León, Mexico.",
    "Biotechnology Department, Dayananda Sagar College of Engineering, Dr. C.D Sagar Centre for Life Sciences, Dayananda Sagar Institutions, Bengaluru, India."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Liyana Nk"
    },
    {
      "affs": [
        0
      ],
      "name": "Tusar Mondal",
      "orcid": "0000-0003-2587-7565"
    },
    {
      "affs": [
        0
      ],
      "name": "Tobika R"
    },
    {
      "affs": [
        0
      ],
      "name": "Kumar Km"
    },
    {
      "affs": [
        1
      ],
      "name": "Yalpi Karthik",
      "orcid": "0000-0002-0097-613X"
    },
    {
      "affs": [
        2
      ],
      "name": "Ramakrishna D"
    },
    {
      "affs": [
        1
      ],
      "name": "Manoj-Kumar Arthikala",
      "orcid": "0000-0002-4535-6524"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/3arsc0wns03nq.bib",
  "cited_by": 0,
  "doi": "10.1099/acmi.0.001200.v3",
  "fulltext_url": "https://europepmc.org/articles/PMC13682339",
  "id": "3arsc0wns03nq",
  "issns": [
    "2516-8290"
  ],
  "landing_url": "https://europepmc.org/article/MED/42903812",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1313|Antibiotic Resistance in Bacteria"
    ]
  ],
  "openalex": "W7141478435",
  "pdf_url": "https://europepmc.org/articles/PMC13682339?pdf=render",
  "pmcid": "PMC13682339",
  "pmid": "42903812",
  "published": "2026-10-20",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/3arsc0wns03nq.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:53Z",
      "native_id": "MED:42903812",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42903812"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:1313|Antibiotic Resistance in Bacteria",
      "subfield": "general"
    }
  ],
  "title": "Genomic analysis and antimicrobial resistance profiling of Klebsiella pneumoniae clinical isolates from urinary tract infections in India: prevalence of class A β-lactamase with molecular docking and simulation insights.",
  "title_text": "Genomic analysis and antimicrobial resistance profiling of Klebsiella pneumoniae clinical isolates from urinary tract infections in India: prevalence of class A β-lactamase with molecular docking and simulation insights.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:53Z",
  "url": "https://research.draconitic.com/paper/3arsc0wns03nq",
  "venue": "Access microbiology",
  "versions": []
}