{
  "abstract": "Themis is important in regulating positive selection of thymocytes during T cell development, but its role in peripheral T cells is less understood. Here, we investigated T cell activation and its sequelae using a tamoxifen-mediated, acute Themis deletion mouse model. We find that proliferation, effector functions including anti-tumor killing, and up-regulation of energy metabolism are severely compromised. This study reveals the phenomenon of peripheral adaptation to loss of Themis, by demonstrating direct TCR-induced defects after acute deletion of Themis that were not evident in peripheral T cells chronically deprived of Themis in dLck-Cre deletion model. Peripheral adaptation to long-term loss was compared using chronic versus acute tamoxifen-mediated deletion and with the (chronic) dLck-Cre deletion model. We found that upon chronic tamoxifen-mediated Themis deletion, there was modulation in the gene expression profile for both TCR and cytokine signaling pathways. This profile overlapped with (chronic) dLck-Cre deletion model. Hence, we found that peripheral adaptation induced changes to both TCR and cytokine signaling modules. Our data highlight the importance of Themis in the activation of CD8 + T cells.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 14,
  "authors": [
    {
      "name": "Namrata Gautam"
    },
    {
      "name": "Lukasz Wojciech"
    },
    {
      "name": "Jiawei Yap"
    },
    {
      "name": "Yen Leong Chua"
    },
    {
      "name": "Eyan Mw Ding"
    },
    {
      "name": "Don Cn Sim"
    },
    {
      "name": "Alrina Sm Tan"
    },
    {
      "name": "Patricia J Ahl"
    },
    {
      "name": "Mukul Prasad"
    },
    {
      "name": "Desmond Wh Tung"
    },
    {
      "name": "John E Connolly"
    },
    {
      "name": "Giulia Adriani"
    },
    {
      "name": "Joanna Brzostek"
    },
    {
      "name": "Nicholas Rj Gascoigne"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/3ayptwqhm0exj.bib",
  "cited_by": 12,
  "doi": "10.26508/lsa.202302156",
  "fulltext_url": null,
  "id": "3ayptwqhm0exj",
  "issns": [
    "2575-1077"
  ],
  "landing_url": "https://hal.science/hal-05776439v1",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "hal",
      "1.sdv.imm"
    ],
    [
      "hal",
      "2.sdv.imm.ia"
    ],
    [
      "openalex",
      "2403|T-cell and B-cell Immunology"
    ]
  ],
  "openalex": "W4386956167",
  "pdf_url": "https://www.life-science-alliance.org/content/lsa/6/12/e202302156.full.pdf",
  "pmcid": null,
  "pmid": "37739454",
  "published": "2026-10-03",
  "publisher": "Life Science Alliance LLC",
  "ris": "https://research.draconitic.com/paper/3ayptwqhm0exj.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:46Z",
      "native_id": "hal-05776439",
      "source": "hal",
      "url": "https://hal.science/hal-05776439v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "hal:1.sdv.imm",
      "subfield": "immunology"
    }
  ],
  "title": "Themis controls T cell activation, effector functions, and metabolism of peripheral CD8+ T cells",
  "title_text": "Themis controls T cell activation, effector functions, and metabolism of peripheral CD8+ T cells",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:46Z",
  "url": "https://research.draconitic.com/paper/3ayptwqhm0exj",
  "venue": "Life Science Alliance",
  "versions": []
}