{
  "abstract": "Here, we present a protocol to generate human organotypic lung cultures from surgical lung resection for the study of respiratory infections. We describe steps for tissue harvesting, biopsy punching, mechanical slicing, and culture at the air-liquid interface. This technique enables robust ex vivo infections of human lung tissues with respiratory viruses, including influenza A and Nipah. It can be used to study host-pathogen interactions, analyze innate immune responses, and evaluate antiviral candidates in physiologically relevant human lung tissue. For complete details on the use and execution of this protocol, please refer to Cezard et al.¹.",
  "abstract_shown": true,
  "affiliations": [
    "CIRI, Centre International de Recherche en Infectiologie, Team NeuroInvasion, Tropism and Viral Encephalitis, University Lyon, Inserm, U1111, CNRS, UMR5308, Université Lyon 1, Ecole Normale Supérieure de Lyon, 69007 Lyon, France.",
    "CIRI, Centre International de Recherche en Infectiologie, Team Viral Infection, Metabolism and Immunity, University Lyon, Inserm, U1111, CNRS, UMR5308, Université Lyon 1, Ecole Normale Supérieure de Lyon, 69007 Lyon, France.",
    "Inserm, U1100, Centre D'Etude des Pathologies Respiratoires (CEPR), University of Tours, 37000 Tours, France.",
    "IVPC, Infections Virales et Pathologie Comparée, UMR754, INRAE, Université Lyon 1, EPHE, 69007 Lyon, France.",
    "Service de Chirurgie Thoracique, CHRU de Tours, Université de Tours, Tours, France.",
    "Service de Pathologie, CHRU de Tours, Tours, France.",
    "Inserm, U1100, Centre D'Etude des Pathologies Respiratoires (CEPR), University of Tours, 37000 Tours, France; Service de Médecine Intensive -Réanimation, CHRU de Tours, Tours, France.",
    "Laboratoire P4 INSERM-Jean Mérieux, Lyon, France.",
    "CIRI, Centre International de Recherche en Infectiologie, Team NeuroInvasion, Tropism and Viral Encephalitis, University Lyon, Inserm, U1111, CNRS, UMR5308, Université Lyon 1, Ecole Normale Supérieure de Lyon, 69007 Lyon, France. Electronic address: cyrille.mathieu@inserm.fr."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 18,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Lola Canus"
    },
    {
      "affs": [
        1
      ],
      "name": "Florentine Jacolin"
    },
    {
      "affs": [
        2
      ],
      "name": "Virginie Vasseur"
    },
    {
      "affs": [
        2
      ],
      "name": "Adeline Cezard"
    },
    {
      "affs": [
        0
      ],
      "name": "Eva Ogire"
    },
    {
      "affs": [
        1
      ],
      "name": "Anne Aublin-Gex"
    },
    {
      "affs": [
        0
      ],
      "name": "Amelie Bourgeais"
    },
    {
      "affs": [
        2
      ],
      "name": "Camille David"
    },
    {
      "affs": [
        3
      ],
      "name": "Alexandra Erny"
    },
    {
      "affs": [
        3
      ],
      "name": "Fabienne Archer"
    },
    {
      "affs": [
        4
      ],
      "name": "Antoine Legras"
    },
    {
      "affs": [
        5
      ],
      "name": "Damien Sizaret"
    },
    {
      "affs": [
        6
      ],
      "name": "Antoine Guillon"
    },
    {
      "affs": [
        7
      ],
      "name": "Vincent Lotteau"
    },
    {
      "affs": [
        1
      ],
      "name": "Pierre-Olivier Vidalain"
    },
    {
      "affs": [
        2
      ],
      "name": "Mustapha Si-Tahar"
    },
    {
      "affs": [
        1
      ],
      "name": "Laure Perrin-Cocon"
    },
    {
      "affs": [
        8
      ],
      "name": "Cyrille Mathieu",
      "orcid": "0000-0002-6682-2029"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/3b4arhbvtcevw.bib",
  "cited_by": 0,
  "doi": "10.1016/j.xpro.2026.104896",
  "fulltext_url": "https://europepmc.org/articles/PMC13686045",
  "id": "3b4arhbvtcevw",
  "issns": [
    "2666-1667"
  ],
  "landing_url": "https://europepmc.org/article/MED/42873646",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2713|Respiratory viral infections research"
    ]
  ],
  "openalex": "W7221712606",
  "pdf_url": "https://europepmc.org/articles/PMC13686045?pdf=render",
  "pmcid": "PMC13686045",
  "pmid": "42873646",
  "published": "2026-10-13",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/3b4arhbvtcevw.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:00Z",
      "native_id": "MED:42873646",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42873646"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2713|Respiratory viral infections research",
      "subfield": "epidemiology-public-health"
    }
  ],
  "title": "Agarose-free protocol for preparing human organotypic lung cultures to study respiratory virus infection and evaluate antivirals ex vivo.",
  "title_text": "Agarose-free protocol for preparing human organotypic lung cultures to study respiratory virus infection and evaluate antivirals ex vivo.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:00Z",
  "url": "https://research.draconitic.com/paper/3b4arhbvtcevw",
  "venue": "STAR protocols",
  "versions": []
}