{
  "abstract": "High-speed atomic force microscopy (HS-AFM) enables visualization of biomolecular dynamics in solution with high spatiotemporal resolution but requires substrates that support stable adsorption without perturbing their structure or dynamics. Mica is widely used as an HS-AFM substrate because it provides an atomically flat surface. Several methods have been proposed to functionalize mica surfaces, but they are not suitable for all biomolecules. Here, we expand the HS-AFM substrate toolbox by introducing methyl- and carboxyl (COOH)-functionalized mica surfaces based on a common silanization strategy. In particular, a COOH-functionalized mica substrate was prepared via a mild thiol-maleimide coupling reaction, enabling efficient surface modification under aqueous conditions. Using proteins with different charges and shapes, we evaluated adsorption and diffusion behaviors on these substrates by HS-AFM. Distinct differences in molecular mobility and binding were observed depending on surface properties. These results provide practical guidelines for substrate selection and broaden the applicability of HS-AFM to diverse biomolecular systems.",
  "abstract_shown": true,
  "affiliations": [
    "Division of Mathematical and Physical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa920-1192, Japan.",
    "Division of Material Chemistry, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa920-1192, Japan.",
    "Division of Nano Life Science, Graduate School of Frontier Science Initiative, Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa920-1192, Japan.",
    "WPI Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa920-1192, Japan.",
    "Faculty of Frontier Engineering, Institute of Science and Engineering, Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa920-1192, Japan.",
    "Department of Bacteriology, Niigata University School of Medicine, 1-757 Asahimachi-dori, Chuo-ku, Niigata951-8510, Japan.",
    "Department of Bacteriology, Osaka Metropolitan University Graduate School of Medicine, 1-4-3 Asahi-Machi, Abeno-ku, Osaka545-8586, Japan.",
    "Division of Research Aids, Hokkaido University Institute for Vaccine Research & Development, Kita 20, Nishi 10, Kita-Ku, Sapporo, Hokkaido001-0020, Japan.",
    "Laboratory of Tuberculosis, Institute of Tropical Disease, Universitas Airlangga, Kampus C JI. Mulyorejo, Surabaya60113, Indonesia."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Kaho Nakamoto"
    },
    {
      "affs": [
        0
      ],
      "name": "Yuna Goto"
    },
    {
      "affs": [
        1
      ],
      "name": "Sohta Yamamoto"
    },
    {
      "affs": [
        2
      ],
      "name": "Hikaru Ichida"
    },
    {
      "affs": [
        3
      ],
      "name": "Kenichi Umeda",
      "orcid": "0000-0002-3650-3828"
    },
    {
      "affs": [
        3,
        4
      ],
      "name": "Keisuke Miyazawa",
      "orcid": "0000-0002-5012-8040"
    },
    {
      "affs": [
        3,
        4
      ],
      "name": "Takeshi Fukuma",
      "orcid": "0000-0001-8971-6002"
    },
    {
      "affs": [
        5
      ],
      "name": "Akihito Nishiyama"
    },
    {
      "affs": [
        5,
        6,
        7,
        8
      ],
      "name": "Sohkichi Matsumoto"
    },
    {
      "affs": [
        1
      ],
      "name": "Hajime Maeda",
      "orcid": "0000-0001-8987-1790"
    },
    {
      "affs": [
        3
      ],
      "name": "Noriyuki Kodera",
      "orcid": "0000-0003-4880-8423"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/3bt9c04yn97df.bib",
  "cited_by": 0,
  "doi": "10.1021/acs.langmuir.6c02731",
  "fulltext_url": "https://europepmc.org/articles/PMC13602910",
  "id": "3bt9c04yn97df",
  "issns": [
    "0743-7463",
    "1520-5827"
  ],
  "landing_url": "https://europepmc.org/article/MED/42917971",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "3107|Force Microscopy Techniques and Applications"
    ]
  ],
  "openalex": "W7208748840",
  "pdf_url": "https://europepmc.org/articles/PMC13602910?pdf=render",
  "pmcid": "PMC13602910",
  "pmid": "42917971",
  "published": "2026-09-01",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/3bt9c04yn97df.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:21:02Z",
      "native_id": "MED:42917971",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42917971"
    }
  ],
  "subjects": [
    {
      "field": "physics",
      "origin": "openalex:3107|Force Microscopy Techniques and Applications",
      "subfield": "amo"
    }
  ],
  "title": "Silane-Based Methyl- and Carboxyl-Functionalized Mica Substrates for High-Speed Atomic Force Microscopy.",
  "title_text": "Silane-Based Methyl- and Carboxyl-Functionalized Mica Substrates for High-Speed Atomic Force Microscopy.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:21:02Z",
  "url": "https://research.draconitic.com/paper/3bt9c04yn97df",
  "venue": "Langmuir : the ACS journal of surfaces and colloids",
  "versions": []
}