{
  "abstract": "Water molecules, as solvents for biomolecules, are essential to cells. The water potential of the cell decreases under water-deficient conditions1,2, yet how cells sense changes in water potential remains unknown. Here we identify a sterile alpha motif (SAM)-containing protein, SAM8, that undergoes water-potential-dependent condensation both in vivo and in vitro and is crucial for hyperosmotic stress tolerance and seed germination. We use biophysical techniques, in vitro reconstitution and bioimaging to demonstrate that SAM8 is strongly hydrated under normal water conditions, preventing its macroscopic condensation. A negatively charged patch determines SAM8 hydration by creating an electric field and micropolar environment. Water-deficient conditions weaken this hydration, thereby activating SAM8 condensation by reprogramming hydrogen bond, electrostatic and hydrophobic interactions. Furthermore, we demonstrate that SAM8 condensates selectively sequester RNA export factors, leading to nuclear retention of mRNAs and translational reprogramming under hyperosmotic stress. Our findings show a mechanism by which plant cells directly sense and respond to water status, shedding light on how they adapt to water deficit conditions. Plants sense water deficiency through SAM8 protein condensation, which responds to reduced hydration and triggers stress adaptation by altering RNA export and gene translation.",
  "abstract_shown": true,
  "affiliations": [
    "Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing, China",
    "State Key Laboratory of Green Biomanufacturing, Beijing, China",
    "Tsinghua University-Peking University Joint Center for Life Sciences, Beijing, China",
    "Zhejiang Key Laboratory of Precise Synthesis of Functional Molecules, Department of Chemistry, Westlake University, Hangzhou, People's Republic of China",
    "State Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, China",
    "Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing, China. xffang@tsinghua.edu.cn",
    "State Key Laboratory of Green Biomanufacturing, Beijing, China. xffang@tsinghua.edu.cn",
    "Tsinghua University-Peking University Joint Center for Life Sciences, Beijing, China. xffang@tsinghua.edu.cn"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Yunhe Wang",
      "orcid": "0000-0003-4908-7664"
    },
    {
      "affs": [
        3
      ],
      "name": "Longchen Zhu"
    },
    {
      "affs": [
        0
      ],
      "name": "Yun Yang"
    },
    {
      "affs": [
        4
      ],
      "name": "Xiaoshuang Li",
      "orcid": "0000-0002-8709-7479"
    },
    {
      "affs": [
        3
      ],
      "name": "Xin Zhang",
      "orcid": "0000-0001-6686-1645"
    },
    {
      "affs": [
        5,
        6,
        7
      ],
      "name": "Xiaofeng Fang",
      "orcid": "0000-0003-1161-9186"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/3d9snhwdnvv4c.bib",
  "cited_by": 14,
  "doi": "10.1038/s41586-026-10591-8",
  "fulltext_url": null,
  "id": "3d9snhwdnvv4c",
  "issns": [
    "0028-0836",
    "1476-4687"
  ],
  "landing_url": "https://doi.org/10.1038/s41586-026-10591-8",
  "language": "en",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1110|Plant Stress Responses and Tolerance"
    ]
  ],
  "openalex": "W7162574932",
  "pdf_url": null,
  "pmcid": "PMC13421324",
  "pmid": "42203875",
  "published": "2026-05-27",
  "publisher": "Nature Portfolio",
  "ris": "https://research.draconitic.com/paper/3d9snhwdnvv4c.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:26:50Z",
      "native_id": "W7162574932",
      "source": "openalex",
      "url": "https://doi.org/10.1038/s41586-026-10591-8"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1110|Plant Stress Responses and Tolerance",
      "subfield": "plant-biology"
    }
  ],
  "title": "Cellular water-potential sensing through biomolecular condensation",
  "title_text": "Cellular water-potential sensing through biomolecular condensation",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T13:21:08Z",
  "url": "https://research.draconitic.com/paper/3d9snhwdnvv4c",
  "venue": "Nature",
  "versions": []
}