{
  "abstract": "Cardiac GSDMD research has largely centred on cardiomyocytes. We quantified donor-level endothelial-minus-cardiomyocyte differences in two single-nucleus cohorts and one GeoMx spatial cohort, retaining assay-specific scales. GSDMD was higher in endothelial compartments in 42/42, 38/38 and 27/32 donors and ranked, post hoc, at the 94.9th, 95.1st and 97.4th percentiles of the corresponding within-cohort signed-contrast distributions. Although positive direction and complete donor concordance were not unique transcriptome-wide, this hypothesis-led target retained high rank across markedly different reference backgrounds and assay modalities. A fixed eight-transcript panel included both endothelial- and cardiomyocyte-shifted members, arguing against a uniform panel-wide directional shift or simple detection failure. GeoMx provided the most discriminating transcriptome background but remained composition-sensitive. GSDMD transcripts are therefore recurrently higher in endothelial than cardiomyocyte compartments across human-heart cohorts, showing that a cardiomyocyte-only interpretation of human-heart GSDMD transcript signals is incomplete.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Internal Medicine, Kyushu University Beppu Hospital, Beppu, Japan."
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "D. Oryoji"
    },
    {
      "name": "G. Doi"
    },
    {
      "name": "S. Fujimoto"
    },
    {
      "name": "N. Nishimura"
    },
    {
      "name": "K. Otsuka"
    },
    {
      "name": "A. Kuwahara"
    },
    {
      "name": "M. Ayano"
    },
    {
      "name": "Y. Kimoto"
    },
    {
      "name": "K. Akashi"
    },
    {
      "name": "H. Niiro"
    },
    {
      "name": "H. Mitoma"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/3dbs8x7h0n3k3.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.06.749635",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.06.749635v1.full",
  "id": "3dbs8x7h0n3k3",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.06.749635v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "cell biology"
    ],
    [
      "openalex",
      "1312|Inflammasome and immune disorders"
    ]
  ],
  "openalex": "W7212119052",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.06.749635v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-06",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/3dbs8x7h0n3k3.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:20:55Z",
      "native_id": "10.64898/2026.09.06.749635",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.06.749635v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:cell biology",
      "subfield": "cell-biology"
    }
  ],
  "title": "GSDMD transcript levels are higher in endothelial than cardiomyocyte compartments across three human heart cohorts",
  "title_text": "GSDMD transcript levels are higher in endothelial than cardiomyocyte compartments across three human heart cohorts",
  "type": "preprint",
  "updated_at": "2026-10-30T12:20:55Z",
  "url": "https://research.draconitic.com/paper/3dbs8x7h0n3k3",
  "venue": "bioRxiv",
  "versions": []
}