{
  "abstract": "5-Azacytidine (5-AzaC) is a cytidine analog chemotherapeutic widely used to treat myeloid malignancies. Although its activity is primarily attributed to DNA hypomethylation, the majority of 5-AzaC is incorporated into RNA, where its functional consequences are less understood. Here, we show that 5-AzaC treatment of cells leads to inhibition of protein synthesis. Ribo-seq, Disome-seq, and RNA-seq in treated cells exhibit a time-dependent C-to-G conversion in mRNAs. These conversion events are enriched within footprint positions corresponding to the A-site of monosomes or stalled leading ribosomes in a disome complex. Footprints are accumulated at sites with C-rich codons in the A-site, with particular emphasis on the second codon position. Consistent with disome-mediated signaling, 5-AzaC activates ZAK- and GCN2-dependent stress responses. Furthermore, loss of the ribosome-associated quality control factor, ZNF598, sensitizes cells to 5-AzaC. These findings suggest that translation disruption represents an additional layer of 5-AzaC’s mechanism of action, alongside its known DNA-mediated effects.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "name": "Alexis B. Roberson"
    },
    {
      "name": "James Marks"
    },
    {
      "name": "Ruby Pitts"
    },
    {
      "name": "Bavavarshini Tamilselvam"
    },
    {
      "name": "Grace D. Britt",
      "orcid": "0009-0002-9395-3603"
    },
    {
      "name": "Brian Grieb"
    },
    {
      "name": "William P. Tansey"
    },
    {
      "name": "Sezen Meydan",
      "orcid": "0000-0002-2504-5489"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/3dk992yr7xf21.bib",
  "cited_by": 0,
  "doi": "10.1016/j.celrep.2026.118247",
  "fulltext_url": "http://www.sciencedirect.com/science/article/pii/S2211124726013264",
  "id": "3dk992yr7xf21",
  "issns": [
    "2211-1247"
  ],
  "landing_url": "https://linkinghub.elsevier.com/retrieve/pii/S2211124726013264",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "http://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "lcc",
      "QH301-705.5"
    ]
  ],
  "openalex": null,
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-11-01",
  "publisher": "Elsevier BV",
  "ris": "https://research.draconitic.com/paper/3dk992yr7xf21.ris",
  "sources": [
    {
      "first_seen": "2026-11-05T00:21:04Z",
      "native_id": "10.1016/j.celrep.2026.118247",
      "source": "crossref",
      "url": "https://linkinghub.elsevier.com/retrieve/pii/S2211124726013264"
    },
    {
      "first_seen": "2026-11-06T14:21:05Z",
      "native_id": "06205beea30348d2be75f83304f03f4c",
      "source": "doaj",
      "url": "https://doi.org/10.1016/j.celrep.2026.118247"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "lcc:QH301-705.5",
      "subfield": "general"
    }
  ],
  "title": "5-Azacytidine incorporation into mRNAs disrupts translation and induces ribosome collisions",
  "title_text": "5-Azacytidine incorporation into mRNAs disrupts translation and induces ribosome collisions",
  "type": "peer-reviewed",
  "updated_at": "2026-11-08T14:20:18Z",
  "url": "https://research.draconitic.com/paper/3dk992yr7xf21",
  "venue": "Cell Reports",
  "versions": []
}