{
  "abstract": "The Polycomb system is an essential regulator of developmental genes which controls chromatin state through histone modifications and 3D chromatin organization. Although Polycomb-mediated histone modification is well understood, how Polycomb-mediated 3D chromatin interactions form remains unclear. To address this gap, we combine comprehensive Polycomb subunit degrons with Region Capture Micro-C, ChIP-seq, nascent transcriptomics, and live-cell imaging of DNA loci and Polycomb bodies. We find that, within canonical Polycomb repressive complex 1 (cPRC1), polyhomeotic proteins are the primary contributors to interactions. In surprising contrast to existing models, we find that Polycomb-mediated interactions persist in the absence of cPRC1, and combined depletion of cPRC1 with individual variant PRC1 and PRC2 complexes indicates that the remaining interactions do not depend on a single complex. These results raise the previously unexplored possibility of a more general mechanism for Polycomb-mediated interactions not requiring cPRC1.",
  "abstract_shown": true,
  "affiliations": [
    "MIT"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "name": "M. K. Huseyin"
    },
    {
      "name": "S. Kim"
    },
    {
      "name": "J. Toppen"
    },
    {
      "name": "N. P. Blackledge"
    },
    {
      "affs": [
        0
      ],
      "name": "A. S. Hansen"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/3e6342kfd4vyk.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.13.758654",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.13.758654v1.full",
  "id": "3e6342kfd4vyk",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.13.758654v1",
  "language": null,
  "licence": "CC-BY-NC",
  "licence_label": "CC BY-NC",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "genomics"
    ],
    [
      "openalex",
      "1312|Genomics and Chromatin Dynamics"
    ]
  ],
  "openalex": "W7221509421",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.13.758654v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-13",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/3e6342kfd4vyk.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:27:51Z",
      "native_id": "10.64898/2026.10.13.758654",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.13.758654v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:genomics",
      "subfield": "genetics-genomics"
    }
  ],
  "title": "Dissection of Polycomb complexes reveals canonical PRC1-independent 3D chromatin interactions",
  "title_text": "Dissection of Polycomb complexes reveals canonical PRC1-independent 3D chromatin interactions",
  "type": "preprint",
  "updated_at": "2026-10-30T12:27:51Z",
  "url": "https://research.draconitic.com/paper/3e6342kfd4vyk",
  "venue": "bioRxiv",
  "versions": []
}