{
  "abstract": "Microbial exopolysaccharides (EPSs) are functional biopolymers of growing scientific and industrial interest because of their structural diversity, biocompatibility, and rheological properties. Microbial β-(1→3)-glucans are of particular biological and technological interest, and their production depends strongly on strain characteristics, medium composition, and culture conditions. This study characterized the temporal relationship among bacterial growth, viability, and aniline-blue reactivity in Alcaligenes faecalis A12C under batch growth and fermentation conditions. Growth turbidity, viable bacterial concentration, and β-(1→3)-glucan-associated signals were monitored over 96 h using spectrophotometric, microbiological, and microscopic approaches. In growth culture medium (GCM), OD600 increased after 24–30 h and reached approximately 2.4 at 72 h, coinciding with the highest viable count (2.44 × 1021 CFU/mL). Aniline-blue coloration became clearly detectable after 30 h and persisted through 96 h, when microscopy showed blue staining associated with bacterial cells. In fermentation culture medium (FCM), OD600 increased progressively to approximately 2.8 at 96 h, whereas viable counts followed a distinct temporal profile. Aniline-blue reactivity changed during the first 24 h and remained close to an A610 of approximately 1.0 thereafter. These findings indicate distinct temporal patterns among culture turbidity, viability, and aniline-blue reactivity and provide a framework for subsequent structural characterization of the detected material.",
  "abstract_shown": true,
  "affiliations": [
    "University Institute of Animal Health and Food Safety (IUSA-ONE HEALTH) 2-Sanidad Animal de la Acuicultura y Especies Silvestres, Enfermedades Infecciosas y Seguridad Alimentaria, University of Las Palmas de Gran Canaria, 35416 Arucas, Spain",
    "Instituto de Investigación Sanitaria de Canarias (IISC), Eukaryotic-Prokaryotic Synergy-Comparative and Translational Medicine Group (Cardiorespiratory Infectious Diseases and Epidemioogy Area), Barranco de la Ballena s/n, 35019 Las Palmas de Gran Canaria, Spain",
    "Fundación Canaria del Instituto de Investigación Sanitaria de Canarias (FIISC), 35010 Las Palmas de Gran Canaria, Spain",
    "Banco Español de Algas, Instituto de Oceanografía y Cambio Global (IOCAG), Universidad de Las Palmas de Gran Canaria, 35214 Telde, Spain",
    "Research Unit, Hospital Universitario de Gran Canaria Dr. Negrín, Fundación del Instituto de Investigación Sanitaria de Canarias (FIISC), 35019 Las Palmas de Gran Canaria, Spain",
    "CIBER de Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, 28029 Madrid, Spain"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 12,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "C. J. Martel-Benítez"
    },
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "R. Henríquez-Cabrera",
      "orcid": "0000-0001-5214-6069"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "R. Alayón-Afonso",
      "orcid": "0000-0002-2928-0144"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "D. Padilla",
      "orcid": "0000-0002-6678-5029"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "F. Real",
      "orcid": "0000-0001-6526-0354"
    },
    {
      "affs": [
        3
      ],
      "name": "A. Martel-Quintana",
      "orcid": "0000-0002-7450-0505"
    },
    {
      "affs": [
        3
      ],
      "name": "J. L. Gómez-Pinchetti",
      "orcid": "0000-0003-4668-0462"
    },
    {
      "affs": [
        3
      ],
      "name": "C. Almeida"
    },
    {
      "affs": [
        3
      ],
      "name": "P. Santiago-Díaz",
      "orcid": "0000-0003-4216-9720"
    },
    {
      "affs": [
        3
      ],
      "name": "N. Robles-Antón",
      "orcid": "0009-0009-1123-4391"
    },
    {
      "affs": [
        3
      ],
      "name": "N. González-Caballero"
    },
    {
      "affs": [
        0,
        1,
        4,
        5
      ],
      "name": "J. L. Martín-Barrasa"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/3fxwznyhnn6vm.bib",
  "cited_by": 0,
  "doi": "10.3390/polym18212749",
  "fulltext_url": null,
  "id": "3fxwznyhnn6vm",
  "issns": [
    "2073-4360"
  ],
  "landing_url": "https://www.mdpi.com/2073-4360/18/21/2749",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [],
  "openalex": null,
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-11-05",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/3fxwznyhnn6vm.ris",
  "sources": [
    {
      "first_seen": "2026-11-05T12:20:55Z",
      "native_id": "10.3390/polym18212749",
      "source": "crossref",
      "url": "https://www.mdpi.com/2073-4360/18/21/2749"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "issn:2073-4360",
      "subfield": "materials-polymers"
    }
  ],
  "title": "Growth Profiles and Temporal Accumulation of Aniline-Blue-Reactive β-(1→3)-Glucan-Associated Material in Alcaligenes faecalis A12C Batch Cultures",
  "title_text": "Growth Profiles and Temporal Accumulation of Aniline-Blue-Reactive β-(1→3)-Glucan-Associated Material in Alcaligenes faecalis A12C Batch Cultures",
  "type": "peer-reviewed",
  "updated_at": "2026-11-07T00:21:08Z",
  "url": "https://research.draconitic.com/paper/3fxwznyhnn6vm",
  "venue": "Polymers",
  "versions": []
}