{
  "abstract": "Nanotechnology has a major impact on the creation of novel and effective therapies for the treatment of several diseases, including diabetes and cancer. The current work is a report on the use of the aqueous extract from the leaves of the Flemingia macrophylla plant as a capping and reducing agent in the green synthesis of zinc oxide nanoparticles (ZnO-NPs).The synthesized ZnO-NPs were subjected to characterization through X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM) scanning electron microscopy (SEM), and ultraviolet-visible spectroscopy (UV-Vis). Dose-dependent in vivo effects were investigated using Drosophila melanogaster subjected to a high-sugar diet (HSD) to induce a diabetic-like model. In vitro anti-oxidant, anti-diabetic, anti-inflammatory assay, survival, locomotor function, neuroanatomical integrity, ovary and testis morphology, egg-laying capacity, and male fertility were evaluated. The most prominent UV-visible peak was detected at around 370 nm. Structural and morphological analysis via XRD and electron microscopy confirmed the hexagonal phase of the ZnO-NPs, indicating an average calcined particle size of around 47 nm at 500 degree C. The manuscript reports significant anti-diabetic and anti-inflammatory effects in vitro and in the in vivo Drosophila model, together with antioxidant activity. The in vivo results indicate that green-synthesized ZnO-NPs mitigate adverse phenotypes triggered by a high-sugar diet and also potential as an anti-diabetic intervention and warrant further mechanistic and preclinical investigation. Keywords: Flemingia macrophylla; Green ZnO-NPs; Drosophila melanogaster; high-sugar diet; nanobiotechnology",
  "abstract_shown": true,
  "affiliations": [
    "Department of Developmental Biology and Genetics (DBG), Indian Institute of Science (IISc)"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "name": "L. Khongsai"
    },
    {
      "name": "R. Sarkar"
    },
    {
      "name": "A. R. Singh"
    },
    {
      "name": "A. S. Singh"
    },
    {
      "name": "B. K. Bindhani"
    },
    {
      "affs": [
        0
      ],
      "name": "U. Nongthomba"
    },
    {
      "name": "C. B. Singh"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/3g31crjezypw9.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.20.760481",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.20.760481v1.full",
  "id": "3g31crjezypw9",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.20.760481v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "pharmacology and toxicology"
    ],
    [
      "openalex",
      "2505|Nanoparticles: synthesis and applications"
    ]
  ],
  "openalex": "W7223563025",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.20.760481v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-20",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/3g31crjezypw9.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:29:38Z",
      "native_id": "10.64898/2026.10.20.760481",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.20.760481v1"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "biorxiv:pharmacology and toxicology",
      "subfield": "pharmacology-toxicology"
    }
  ],
  "title": "Bio-Templated Zinc Oxide Nanotherapeutics from Flemingia macrophylla kuntze Ex Merr: In Vivo Anti-Diabetic and Anti-Inflammatory Efficacy in Drosophila melanogaster",
  "title_text": "Bio-Templated Zinc Oxide Nanotherapeutics from Flemingia macrophylla kuntze Ex Merr: In Vivo Anti-Diabetic and Anti-Inflammatory Efficacy in Drosophila melanogaster",
  "type": "preprint",
  "updated_at": "2026-10-30T12:29:38Z",
  "url": "https://research.draconitic.com/paper/3g31crjezypw9",
  "venue": "bioRxiv",
  "versions": []
}