{
  "abstract": "The high-amylose maize (Zea mays L.) germplasm line GEMS-0067 carries the recessive amylose-extender (ae/sbe2b) allele together with modifier loci that elevate starch amylose content from approximately 55% to more than 70%. Previous quantitative trait locus (QTL) studies associated a major component of this modifier effect with the Sbe1a region on chromosome 5S; however, the underlying DNA polymorphism and practical breeding markers remained unresolved. To identify candidate variation, a targeted genomic amplicon-scanning strategy was applied across coding and non-coding regions of Sbe1a. Screening with 23 gene-specific primer pairs identified a unique fragment-length polymorphism spanning the intron 8-exon 9 boundary. Sequencing revealed a 6 bp TACATA insertion together with a downstream 2 bp GA insertion within intron 8. The polymorphism was present in GEMS-0067 and its ancestral source Guatemala 209 but absent from 33 surveyed USDA Germplasm Enhancement of Maize (GEM) releases. Native PAGE zymogram analysis identified a distinct starch-metabolizing enzyme activity band in the putative SBEI region that displayed altered migration in GEMS-0067 relative to reference genotypes. A PCR-CAPS marker based on the insertion was developed using MslI digestion and enabled clear codominant discrimination of homozygous and heterozygous individuals. These results establish a novel gene-based marker associated with the GEMS-0067 high-amylose modifier background and provide a practical tool for marker-assisted breeding.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Agricultural Research and Development Program, Central State University, Wilberforce, OH 45384, USA.",
    "Syngenta Seeds, DeKalb, IL 60150, USA.",
    "Department of Agronomy, Iowa State University, Ames, IA 50011, USA.",
    "United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Iowa State University, Ames, IA 50011, USA."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Mark R Campbell"
    },
    {
      "affs": [
        1
      ],
      "name": "Avinash Karn",
      "orcid": "0000-0001-9730-8922"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Marvin Paul Scott"
    },
    {
      "affs": [
        0
      ],
      "name": "Luke A Farno"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/3h9w03vxjk55c.bib",
  "cited_by": 0,
  "doi": "10.3390/plants15203157",
  "fulltext_url": "https://europepmc.org/articles/PMC13709310",
  "id": "3h9w03vxjk55c",
  "issns": [
    "2223-7747"
  ],
  "landing_url": "https://europepmc.org/article/MED/42943110",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1311|Genetic Mapping and Diversity in Plants and Animals"
    ]
  ],
  "openalex": "W7222072285",
  "pdf_url": "https://europepmc.org/articles/PMC13709310?pdf=render",
  "pmcid": "PMC13709310",
  "pmid": "42943110",
  "published": "2026-10-15",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/3h9w03vxjk55c.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:22:12Z",
      "native_id": "MED:42943110",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42943110"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1311|Genetic Mapping and Diversity in Plants and Animals",
      "subfield": "genetics-genomics"
    }
  ],
  "title": "The High-Amylose Maize Line GEMS-0067 Is Associated with an Intron 8 Insertion in Sbe1a, Altered Native PAGE Zymogram Patterns, and Development of PCR Markers for Breeding Applications.",
  "title_text": "The High-Amylose Maize Line GEMS-0067 Is Associated with an Intron 8 Insertion in Sbe1a, Altered Native PAGE Zymogram Patterns, and Development of PCR Markers for Breeding Applications.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:22:12Z",
  "url": "https://research.draconitic.com/paper/3h9w03vxjk55c",
  "venue": "Plants (Basel, Switzerland)",
  "versions": []
}