{
  "abstract": "High-risk HPV is central to cervical carcinogenesis, but whether individual genotypes drive distinct tumor biology or converge on a shared oncogenic state remains unclear. Profiling viral and host gene expression across two independent cohorts (n = 93; 76 HPV16, 17 HPV18), we show that HPV16- and HPV18-associated cervical cancers differ in host transcriptional programs. A 55-gene signature discriminated the two genotypes, and 293 genes were concordantly differentially expressed across cohorts. Pathway and transcription-factor activity inference organized these differences along genotype-specific axes: HPV16 tumors were enriched for epithelial polarity and RTK-RAS-MAPK signatures, with elevated inferred CDX2 regulon activity, a transcription factor not normally active in cervical epithelium, suggesting an aberrant lineage-identity program not previously linked to this genotype. HPV18 tumors instead showed metabolic reprogramming and inflammatory signaling (STAT3, HIF1A), alongside reduced CASP8 and increased FDX1 expression, a pattern consistent with sensitization to immunogenic cell death. Both genotypes also organized along a continuum of early-to-late viral gene expression correlating with distinct host pathways: extracellular-matrix and translation-related in HPV16, interferon/antiviral and cell-cycle/mitochondrial in HPV18, indicating that viral transcriptional state associates with host biology beyond genotype alone. These results point to candidate genotype-specific vulnerabilities that merit functional validation.",
  "abstract_shown": true,
  "affiliations": [
    "Universidade de Sao Paulo"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 13,
  "authors": [
    {
      "name": "M. A. Ishihara"
    },
    {
      "name": "M. M. Moro Valeriano"
    },
    {
      "name": "N. Oliveira"
    },
    {
      "name": "I. Mamede"
    },
    {
      "name": "L. Cavalcanti de Morais Gama"
    },
    {
      "name": "A. Ferreira Cunha"
    },
    {
      "name": "I. Maia Santos de Castro"
    },
    {
      "name": "F. Vasquez"
    },
    {
      "name": "M. Y. Nishiyama-Jr"
    },
    {
      "name": "J. C. Setubal"
    },
    {
      "name": "E. de Arruda Neto"
    },
    {
      "name": "R. Chammas"
    },
    {
      "affs": [
        0
      ],
      "name": "H. I. Nakaya"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/3jangv1a26xy8.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.30.755713",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.30.755713v1.full",
  "id": "3jangv1a26xy8",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.30.755713v1",
  "language": null,
  "licence": "CC-BY-NC",
  "licence_label": "CC BY-NC",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "cancer biology"
    ],
    [
      "openalex",
      "2713|Cervical Cancer and HPV Research"
    ]
  ],
  "openalex": "W7215896588",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.30.755713v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-30",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/3jangv1a26xy8.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:25:46Z",
      "native_id": "10.64898/2026.09.30.755713",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.30.755713v1"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "biorxiv:cancer biology",
      "subfield": "oncology"
    }
  ],
  "title": "Multi-scale Transcriptional Characterization of HPV16 and HPV18 in Cervical Cancer: Host Pathway Alterations and Links With Viral Gene Expression Programs",
  "title_text": "Multi-scale Transcriptional Characterization of HPV16 and HPV18 in Cervical Cancer: Host Pathway Alterations and Links With Viral Gene Expression Programs",
  "type": "preprint",
  "updated_at": "2026-10-30T12:25:46Z",
  "url": "https://research.draconitic.com/paper/3jangv1a26xy8",
  "venue": "bioRxiv",
  "versions": []
}